Zheng Jianyu, Tian Qing, Hou Weihong, Watts John A, Schrum Laura W, Bonkovsky Herbert L
Liver-Biliary-Pancreatic Center, Carolinas Medical Center, 1000 Blythe Boulevard, Charlotte, NC 28203, USA.
FEBS Lett. 2008 Jun 11;582(13):1829-34. doi: 10.1016/j.febslet.2008.04.047. Epub 2008 May 8.
5-Aminolevulinic acid synthase-1 (ALAS1) and heme oxygenase-1 (HO-1) are the rate-controlling enzymes for heme biosynthesis and degradation, respectively. Expression of these two genes showed tissue-specific expression pattern at both mRNA and protein levels in selected non-treated rat tissues. In the livers of rats receiving oral ethanol for 10 weeks, ALAS1 mRNA levels were increased by 65%, and the precursor and mature ALAS1 protein levels were increased by 1.8- and 2.3-fold, respectively, while no changes were observed in HO-1 mRNA and protein levels, compared with pair-fed controls. These results provide novel insights into the effects of chronic ethanol consumption on hepatic heme biosynthesis and porphyrias.
5-氨基酮戊酸合酶-1（ALAS1）和血红素加氧酶-1（HO-1）分别是血红素生物合成和降解的限速酶。在选定的未处理大鼠组织中，这两个基因的表达在mRNA和蛋白质水平上均呈现组织特异性表达模式。与配对喂养的对照组相比，在接受口服乙醇10周的大鼠肝脏中，ALAS1 mRNA水平增加了65%，ALAS1前体和成熟蛋白水平分别增加了1.8倍和2.3倍，而HO-1 mRNA和蛋白质水平未观察到变化。这些结果为慢性乙醇摄入对肝脏血红素生物合成和卟啉症的影响提供了新的见解。
