硫酸几丁聚糖可调节 HepG2 细胞的蛋白质合成，并增强 NF-κB 和 C/EBP 结合活性。
Curdlan sulphate modulates protein synthesis and enhances NF-kappaB and C/EBP binding activity in HepG2 cells.
机构信息
Institute of Molecular Biology Jagiellonian University Al. Mickiewicza 3 Krakow 31-120 Poland.
出版信息
Mediators Inflamm. 1997;6(1):58-63. doi: 10.1080/09629359791947.
In human hepatoma HepG2 cell line curdlan sulphate enhances basal and interleukin-6-stimulated fibrinogen and antichymotrypsin (ACT) synthesis, slightly increases basal ceruloplasmin production and exerts only minor effects on alpha-1-proteinase inhibitor and transferrin. Curdlan sulphate may, at least in part, affect protein synthesis at a pretranslational level, as the expression of ACT mRNA was found to be increased, whereas intracellular enzyme, manganese superoxide dismutase mRNA level was decreased in the cell culture treated with curdlan sulphate. Gel mobility shift analysis revealed that curdlan sulphate increases the DNA binding activity of NF-kappaB and C/EBP, suggesting that these transcription factors may participate in the regulatory effects of curdlan sulphate in HepG2 cells.
在人肝癌 HepG2 细胞系中，昆布多糖硫酸盐增强基础状态和白细胞介素-6 刺激的纤维蛋白原和抗胰蛋白酶（ACT）合成，略微增加基础铜蓝蛋白的产生，并且对α-1-蛋白酶抑制剂和转铁蛋白只有很小的影响。昆布多糖硫酸盐可能至少部分地在翻译前水平上影响蛋白质合成，因为发现细胞培养物中 ACT mRNA 的表达增加，而细胞内酶，锰超氧化物歧化酶 mRNA 水平降低。凝胶迁移率变动分析显示，昆布多糖硫酸盐增加 NF-κB 和 C/EBP 的 DNA 结合活性，表明这些转录因子可能参与昆布多糖硫酸盐对 HepG2 细胞的调节作用。
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