Maeda Y Y, Takahama S, Kohara Y, Yonekawa H
Department of Laboratory Animal Science, The Tokyo Metropolitan Institute of Medical Science, Tokyo 113, Japan.
Immunogenetics. 1996;43(4):215-9. doi: 10.1007/BF00587302.
Lentinan, a beta-1,6;1,3-glucan, is tumor-specific for transplantable mouse solid-type tumors and it also stimulates the production of acute phase proteins (APPs). The APP response to lentinan is of the delayed type (DT-APR) and differs from that to lipopolysaccharide, which is acute. We found that the responses were genetically controlled in mice and that low responsiveness is dominant (Maeda et al. 1991). Using 123 segregants of crosses between SWR/J (a high responder) and Mus spretus (a low responder), we analyzed the linkage between DT-APR responsiveness and the DNA polymerase chain reaction-simple sequence length polymorphism (PCR-SSLP) phenotype using 80 chromosome-specific microsatellite markers. We identified two loci (ltn1.1 and ltn1.2) responsible for DT-APR. ltn1.1 is closely linked to D3Mit11 on chromosome 3 and ltn1.2 to D11Nds9 on chromosome 11 (P <0.001). The linkage analysis also suggested that ltn1.2 is the major determinant for DT-APR. Correlation between lentinan-specific IL-6 mRNA expression (the late expression) controlled recessively and DT-APR induction suggests that the ltn1 loci control some process(es) of IL-6 expression in the regulation step before NF-IL6.
香菇多糖是一种β-1,6;1,3-葡聚糖，对可移植的小鼠实体瘤具有肿瘤特异性，并且还能刺激急性期蛋白（APPs）的产生。对香菇多糖的APP反应属于延迟型（DT-APR），与对脂多糖的急性反应不同。我们发现小鼠的这些反应受基因控制，低反应性为显性（前田等人，1991年）。利用SWR/J（高反应者）和小家鼠（低反应者）杂交产生的123个分离株，我们使用80个染色体特异性微卫星标记分析了DT-APR反应性与DNA聚合酶链反应-简单序列长度多态性（PCR-SSLP）表型之间的连锁关系。我们确定了两个负责DT-APR的基因座（ltn1.1和ltn1.2）。ltn1.1与3号染色体上的D3Mit11紧密连锁，ltn1.2与11号染色体上的D11Nds9紧密连锁（P<0.001）。连锁分析还表明ltn1.2是DT-APR的主要决定因素。隐性控制的香菇多糖特异性IL-6 mRNA表达（晚期表达）与DT-APR诱导之间的相关性表明，ltn1基因座在NF-IL6之前的调节步骤中控制IL-6表达的某些过程。
