Weiner Michael, Slatko Barton
Affomix Corporation, New Haven, CT 06511, USA.
Biotechniques. 2008 Apr;44(5):701-4. doi: 10.2144/000112796.
Since their initial development nearly 20 years ago, molecular biology kits have evolved from simple protocols and reagents for cloning of DNA to the more recent complex reagent sets that enable whole genomic sequencing. Initially met with resistance by some who felt that using them deprived researchers of the basic knowledge of how to create reagents, molecular biology kits have taken on an important role in the biological sciences. In this article we describe kit development, why kits have succeeded in molecular biology, and how they have paved the way for the more recent widespread use of core facilities.
自近20年前首次开发以来，分子生物学试剂盒已从用于DNA克隆的简单方案和试剂发展到能够进行全基因组测序的最新复杂试剂套装。最初，一些人对它们持抵制态度，认为使用试剂盒剥夺了研究人员关于如何制备试剂的基本知识，但分子生物学试剂盒如今已在生物科学领域发挥了重要作用。在本文中，我们将描述试剂盒的发展、试剂盒在分子生物学中取得成功的原因，以及它们如何为核心设施最近的广泛应用铺平了道路。
