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分离出一种马立克氏病病毒（MDV）重组体，其含有稳定插入MDV US2基因内的大肠杆菌lacZ基因。

Isolation of a Marek's disease virus (MDV) recombinant containing the lacZ gene of Escherichia coli stably inserted within the MDV US2 gene.

作者信息

Cantello J L, Anderson A S, Francesconi A, Morgan R W

机构信息

Delaware Agricultural Experiment Station, Department of Animal Science and Agricultural Biochemistry, College of Agricultural Sciences, University of Delaware, Newark 19717-1303.

出版信息

J Virol. 1991 Mar;65(3):1584-8. doi: 10.1128/JVI.65.3.1584-1588.1991.

DOI:10.1128/JVI.65.3.1584-1588.1991
PMID:1847475
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC239942/
Abstract

We have isolated a stable, recombinant Marek's disease virus (MDV) containing the lacZ gene of Escherichia coli inserted into the unique short region of the genome. The nucleotide sequence of the insertion site indicates that it lies within a sequence homologous to the US2 gene of herpes simplex virus. Stable insertion of the lacZ gene into the MDV US2 gene indicates that the site is nonessential for MDV growth in cell culture.

摘要

我们已经分离出一种稳定的重组马立克氏病病毒（MDV），其基因组的独特短区域插入了大肠杆菌的lacZ基因。插入位点的核苷酸序列表明，它位于与单纯疱疹病毒US2基因同源的序列内。lacZ基因稳定插入MDV US2基因表明该位点对于MDV在细胞培养中的生长并非必需。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/907f/239942/92887eee13a4/jvirol00046-0546-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/907f/239942/92887eee13a4/jvirol00046-0546-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/907f/239942/92887eee13a4/jvirol00046-0546-a.jpg

相似文献

1
Isolation of a Marek's disease virus (MDV) recombinant containing the lacZ gene of Escherichia coli stably inserted within the MDV US2 gene.分离出一种马立克氏病病毒（MDV）重组体，其含有稳定插入MDV US2基因内的大肠杆菌lacZ基因。
J Virol. 1991 Mar;65(3):1584-8. doi: 10.1128/JVI.65.3.1584-1588.1991.
2
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本文引用的文献

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Field trials to test the efficacy of polyvalent Marek's disease vaccines in broilers.测试多价马立克氏病疫苗对肉鸡有效性的田间试验。
Avian Dis. 1984 Jan-Mar;28(1):44-60.
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Field trials with a bivalent vaccine (HVT and SB-1) against Marek's disease.使用二价疫苗（禽疱疹病毒疫苗和SB-1）预防马立克氏病的田间试验。
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在火鸡疱疹病毒 US2 基因插入位点表达 HPAI H5N1 病毒的 HA 比在 US10 基因插入位点表达的 HA 诱导更好的保护效果。
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Reconstitution of Marek's disease virus serotype 1 (MDV-1) from DNA cloned as a bacterial artificial chromosome and characterization of a glycoprotein B-negative MDV-1 mutant.从作为细菌人工染色体克隆的DNA中重建1型马立克氏病病毒（MDV-1）以及糖蛋白B阴性MDV-1突变体的特性分析
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Development of an effective polyvalent vaccine against both Marek's and Newcastle diseases based on recombinant Marek's disease virus type 1 in commercial chickens with maternal antibodies.基于重组1型马立克氏病病毒，在具有母源抗体的商品鸡中开发一种针对马立克氏病和新城疫的有效多价疫苗。
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The equine herpesvirus 1 Us2 homolog encodes a nonessential membrane-associated virion component.马疱疹病毒1型的Us2同源物编码一种非必需的膜相关病毒粒子成分。
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Recombinant Marek's disease virus (MDV)-derived lymphoblastoid cell lines: regulation of a marker gene within the context of the MDV genome.重组马立克氏病病毒（MDV）衍生的淋巴母细胞系：MDV基因组背景下标记基因的调控
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The glycoprotein D (US6) homolog is not essential for oncogenicity or horizontal transmission of Marek's disease virus.糖蛋白D（US6）同源物对于马立克氏病病毒的致癌性或水平传播并非必不可少。
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Alterations in DNA sequence and RNA transcription of the Bam HI-H fragment accompany attenuation of oncogenic Marek's disease herpesvirus.BamHI-H片段的DNA序列和RNA转录的改变伴随着致癌性马立克氏病疱疹病毒的减毒。
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Protection against Marek's disease by vaccination with a herpesvirus of turkeys.用火鸡疱疹病毒疫苗预防马立克氏病。
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A herpesvirus isolated from kidney cell culture of normal turkeys.从正常火鸡肾细胞培养物中分离出的一种疱疹病毒。
Avian Dis. 1969 Nov;13(4):853-63.
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Field trials with the herpes virus of turkeys (HVT) strain FC126 as a vaccine against Marek's disease.
Poult Sci. 1971 May;50(3):775-83. doi: 10.3382/ps.0500775.
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Isolation from turkeys of a cell-associated herpesvirus antigenically related to Marek's disease virus.从火鸡中分离出一种与马立克氏病病毒抗原相关的细胞相关疱疹病毒。
Am J Vet Res. 1970 Mar;31(3):525-38.
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Agent of Marek's disease in tissue culture.马立克氏病病毒在组织培养中的作用因子。
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Vaccinia virus expression vector: coexpression of beta-galactosidase provides visual screening of recombinant virus plaques.痘苗病毒表达载体：β-半乳糖苷酶的共表达可对重组病毒噬斑进行可视化筛选。
Mol Cell Biol. 1985 Dec;5(12):3403-9. doi: 10.1128/mcb.5.12.3403-3409.1985.
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Vaccinia virus vectors utilizing the beta-galactosidase assay for rapid selection of recombinant viruses and measurement of gene expression.利用β-半乳糖苷酶测定法快速筛选重组病毒并测量基因表达的痘苗病毒载体。
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