分离出一种马立克氏病病毒（MDV）重组体，其含有稳定插入MDV US2基因内的大肠杆菌lacZ基因。
Isolation of a Marek's disease virus (MDV) recombinant containing the lacZ gene of Escherichia coli stably inserted within the MDV US2 gene.
作者信息
Cantello J L, Anderson A S, Francesconi A, Morgan R W
机构信息
Delaware Agricultural Experiment Station, Department of Animal Science and Agricultural Biochemistry, College of Agricultural Sciences, University of Delaware, Newark 19717-1303.
出版信息
J Virol. 1991 Mar;65(3):1584-8. doi: 10.1128/JVI.65.3.1584-1588.1991.
We have isolated a stable, recombinant Marek's disease virus (MDV) containing the lacZ gene of Escherichia coli inserted into the unique short region of the genome. The nucleotide sequence of the insertion site indicates that it lies within a sequence homologous to the US2 gene of herpes simplex virus. Stable insertion of the lacZ gene into the MDV US2 gene indicates that the site is nonessential for MDV growth in cell culture.
我们已经分离出一种稳定的重组马立克氏病病毒（MDV），其基因组的独特短区域插入了大肠杆菌的lacZ基因。插入位点的核苷酸序列表明，它位于与单纯疱疹病毒US2基因同源的序列内。lacZ基因稳定插入MDV US2基因表明该位点对于MDV在细胞培养中的生长并非必需。
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