Centre of Internal Medicine Philipps-University Baldinger Strasse Marburg D-355 Germany.
Mediators Inflamm. 1992;1(1):61-6. doi: 10.1155/S0962935192000127.
Cuprophane membranes during haemodialysis significantly increase the plasma levels of C5a(desArg) (maximal 55 mug C5a(adesArg)/1 blood after 30 min) whereas Hemophane or Polysulphonemembranes induce only low plasma levels of C5a(desArg). C5a(desArg) generated in vitro by yeast incubation of autologous plasma stimulates PMN chemotaxis and oxidative metabolism but has no effect on enzyme release. Preincubation of whole blood with C5a(desArg) causes aggregation and changed oxidative burst activity of the isolated PMN. These changes are similar to those found in cells from patients after haemodialysis with cuprophane membranes. So the elevated plasma levels of C5a(desArg) after haemodialysis explain some of the changes in PMN functions, but additional mechanisms have to be assumed.
铜仿膜在血液透析过程中可显著增加 C5a(desArg)的血浆水平(30 分钟后最大可达到 55 微克 C5a(adesArg)/1 血),而血仿膜或聚砜膜仅引起 C5a(desArg)的低血浆水平。酵母孵育自身血浆产生的 C5a(desArg)在体外可刺激 PMN 的趋化性和氧化代谢,但对酶释放没有影响。全血与 C5a(desArg)预孵育可引起分离 PMN 的聚集和氧化爆发活性改变。这些变化与铜仿膜血液透析后患者细胞中发现的变化相似。因此,血液透析后 C5a(desArg)的血浆水平升高可解释 PMN 功能的一些变化,但需要假设其他机制。