Mario Negri Institute for Pharmacological Research via Gavazzeni 11 Bergamo Italy.
Mediators Inflamm. 1993;2(6):429-33. doi: 10.1155/S0962935193000614.
The effect of interleukin-6 (IL-6) on gene expression of extracellular matrix components in bovine mesangial cells in culture has been investigated. IL-6 (100 U/ml) time dependently increased the steady state expression of mRNAs coding for alpha1 collagen III and fibronectin, both transcripts being 1.5- and 2.5-fold higher than basal level at 24 and 48 h, respectively. In contrast, IL-6 stimulated laminin mRNA expression only after 48 h incubation (2.5-fold upon basal level). These results suggest that IL-6 could favour glomerular matrix accumulation thus contributing to the development of glomerulosclerosis.
本研究旨在探讨白细胞介素 6(IL-6)对培养的牛肾小球系膜细胞细胞外基质成分基因表达的影响。结果表明,IL-6(100U/ml)可使编码α1 胶原 III 和纤维连接蛋白的 mRNA 转录本的稳态表达水平呈时间依赖性增加,在 24 和 48 小时时,分别比基础水平高 1.5 倍和 2.5 倍。相反,IL-6 仅在 48 小时孵育后才刺激层粘连蛋白 mRNA 的表达(比基础水平增加 2.5 倍)。这些结果提示,IL-6 可能有利于肾小球基质的积累,从而有助于肾小球硬化的发展。