Rambaldi A, Young D C, Griffin J D
Blood. 1987 May;69(5):1409-13.
Monocyte colony-stimulating factor (M-CSF, CSF-1) is a macrophage lineage-specific growth factor. Northern blot analysis using a human M-CSF cDNA probe and a specific bioassay for human M-CSF were used to investigate the cellular sources of M-CSF. Expression of the M-CSF gene was induced in blood mononuclear cells stimulated by phorbol myristate acetate (PMA) or gamma-interferon. When mononuclear cells were fractionated into highly purified populations of T cells and monocytes, M-CSF transcripts were detected predominantly in monocytes. Further, monocytes stimulated with PMA released an M-CSF-like activity. These results demonstrate that M-CSF can be produced by activated cells of the macrophage lineage.
单核细胞集落刺激因子（M-CSF，CSF-1）是一种巨噬细胞谱系特异性生长因子。使用人M-CSF cDNA探针进行的Northern印迹分析以及针对人M-CSF的特异性生物测定法，用于研究M-CSF的细胞来源。佛波酯（PMA）或γ-干扰素刺激的血液单核细胞中，M-CSF基因的表达被诱导。当单核细胞被分离成高度纯化的T细胞和单核细胞群体时，M-CSF转录本主要在单核细胞中被检测到。此外，用PMA刺激的单核细胞释放出一种M-CSF样活性。这些结果表明，M-CSF可由巨噬细胞谱系的活化细胞产生。
