Department of Periodontology and Endodontology School of Dentistry the University of Tokushima 3 Kuramoto-cho Tokushima 770 Japan.
Mediators Inflamm. 1995;4(2):95-7. doi: 10.1155/S0962935195000159.
Fourteen kilodalton phospholipase A(2) molecules (PLA(2)) are classified into two groups, I- and II-PLA(2), and only the latter has been considered to play a pathogenetic role in various forms of tissue inflammation. Previously we demonstrated high PLA(2) activity in gingival crevicular fluid (GCF) of patients with periodontal disease, without determining the group of the enzyme involved. In this study, the activity, groups and levels of enzyme in gingiva taken from 13 sites of periodontal disease were determined using both biochemical and radioimmunological methods. A linear correlation between the activity and the level of II-PLA(2) was observed. No I-PLA(2) was found in any of the samples tested. These data suggest that the PLA(2) activity found in the GCF of patients with periodontal disease does not belong to the I-PLA(2) but to the II-PLA(2) group.
十四千道尔顿的磷脂酶 A(2)分子(PLA(2))分为两组,I 型和 II 型 PLA(2),只有后者被认为在各种形式的组织炎症中起致病作用。以前我们已经证明在牙周病患者的龈沟液(GCF)中存在高 PLA(2)活性,但是没有确定涉及的酶的组别。在这项研究中,使用生化和放射免疫方法测定了取自牙周病 13 个部位的牙龈中的酶的活性、组别和水平。观察到活性和 II 型 PLA(2)水平之间存在线性相关性。在测试的任何样本中都没有发现 I 型 PLA(2)。这些数据表明,在牙周病患者的 GCF 中发现的 PLA(2)活性不属于 I 型 PLA(2),而属于 II 型 PLA(2)组。