Eguchi S, Kozuka M, Hirose S, Ito T, Hirata Y, Marumo F, Hagiwara H
Department of Biological Sciences, Tokyo Institute of Technology, Japan.
Biochem Biophys Res Commun. 1991 Feb 14;174(3):1343-6. doi: 10.1016/0006-291x(91)91570-3.
Autocrine and paracrine regulation of vascular endothelial cells by endothelins has been postulated and has been the target of many recent investigations. In the present study, we demonstrated, by affinity labeling, the presence of endothelin-1- and endothelin-3-specific receptors on cultured bovine endothelial cells that secrete endothelin; the endothelin-3 receptor was the major form. Analysis using the GTP analogue guanosine 5'-O- (thiotriphosphate) indicated that these receptors are coupled to G proteins.
内皮素对血管内皮细胞的自分泌和旁分泌调节已被提出，并且是近期许多研究的目标。在本研究中，我们通过亲和标记证明，在分泌内皮素的培养牛内皮细胞上存在内皮素-1和内皮素-3特异性受体；内皮素-3受体是主要形式。使用鸟苷5'-O-（硫代三磷酸）这种GTP类似物进行的分析表明，这些受体与G蛋白偶联。
