El-Saharty Yasser Shaker Ibrahim
King Faisal University, Faculty of Clinical Pharmacy, Department of Pharmaceutical Analytical Chemistry, Al-Hafhouf, PO Box 400, Kingdom of Saudi Arabia.
J AOAC Int. 2008 Mar-Apr;91(2):311-21.
A mixture of piracetam and vincamine was determined by 3 different methods. The first was the determination of piracetam and vincamine using the ratio-spectra first-derivative (DD1) spectrophotometric technique at 209 and 293 nm in concentration ranges of 10-45 and 2-14 microg/mL with mean recoveries of 99.22 +/- 0.72 and 99.67 +/- 0.79%, respectively. The second method was based on the resolution of the 2 components by bivariate calibration depending on a mathematic algorithm that provides simplicity and rapidity. The method depended on quantitative evaluation of the absorbencies at 210 and 225 nm in concentration ranges of 5-45 and 2-14 microg/mL, with mean recoveries of 100.33 +/- 0.54 and 100.44 +/- 0.98% for piracetam and vincamine, respectively. The third method was reversed-phase liquid chromatography using 0.05 M potassium dihydrogen phosphate-methanol (50 + 50, v/v) as the mobile phase, with the pH adjusted to 3.5 with phosphoric acid. The eluent was monitored at 215 nm in concentration ranges of 5-100 and 2-200 microg/mL, with mean recoveries of 99.62 +/- 0.67 and 99.32 +/- 0.85% for piracetam and vincamine, respectively. The suggested procedures were checked using laboratory-prepared mixtures and were successfully applied for the analysis of their pharmaceutical preparation. The methods retained their accuracy and precision when applying the standard addition technique. The results obtained by applying the proposed methods were statistically analyzed and compared with those obtained by the manufacturer's method.
采用3种不同方法对吡拉西坦和长春胺的混合物进行了测定。第一种方法是使用比率光谱一阶导数（DD1）分光光度技术在209和293nm处测定吡拉西坦和长春胺，浓度范围分别为10 - 45μg/mL和2 - 14μg/mL，平均回收率分别为99.22±0.72%和99.67±0.79%。第二种方法基于双变量校准解析这两种成分，该方法依赖于一种提供简便性和快速性的数学算法。该方法通过在210和225nm处对吸光度进行定量评估，吡拉西坦和长春胺的浓度范围分别为5 - 45μg/mL和2 - 14μg/mL，平均回收率分别为100.33±0.54%和100.44±0.98%。第三种方法是反相液相色谱法，使用0.05M磷酸二氢钾 - 甲醇（50 + 50，v/v）作为流动相，用磷酸将pH值调至3.5。在215nm处监测洗脱液，吡拉西坦和长春胺的浓度范围分别为5 - 100μg/mL和2 - 200μg/mL，平均回收率分别为99.62±0.67%和99.32±0.85%。使用实验室配制的混合物对所建议的方法进行了检验，并成功应用于其药物制剂的分析。当应用标准加入技术时，这些方法保持了其准确性和精密度。对应用所提出方法获得的结果进行了统计分析，并与制造商方法获得的结果进行了比较。
