一种用于定量止泻木（库尔奇）中锥丝碱的可见吸收光密度法的开发与验证

Development and validation of a visible absorption densitometry method for quantitation of conessine in Holarrhena antidysenterica (Kurchi).

作者信息

Patel Rakesh K, Prajapati Arun M

机构信息

Ganpat University, S.K. Patel College of Pharmaceutical Education and Research, Kherava-382 711, Mehsana, Gujarat, India.

出版信息

J AOAC Int. 2008 Mar-Apr;91(2):339-43.

PMID:18476345

Abstract

A selective, precise, and accurate high-performance thin-layer chromatographic (HPTLC) method has been proposed for the analysis of conessine in Holarrhena antidysenterica. The method involves visible densitometric evaluation of conessine resolving it by HPTLC on aluminium-based silica gel plates. For visible densitometric evaluation, peak areas were recorded at 520 nm after the resolved bands were derivatized with Dragendorff's reagent and then sprayed with a 10% solution of aqueous sodium nitrite which resulted in reddish brown color. The correlation between the concentration and area was found to be linear within the range of 10 to 60 ng/spot. The method was validated for precision (interday and intraday), repeatability, and accuracy. Mean recovery for conessine was 98.34-100.25%. The method was applied for the quantitation of conessine in Kurchi. The proposed HPTLC method was found to be precise, specific, sensitive, and accurate and can be used for routine analysis of Kurchi.

摘要

已提出一种选择性、精确且准确的高效薄层色谱（HPTLC）方法，用于分析止泻木中的康丝碱。该方法包括通过在铝基硅胶板上进行HPTLC分离康丝碱后，对其进行可见密度测定评估。对于可见密度测定评估，在用碘化铋钾试剂衍生分离后的条带，然后喷上10%的亚硝酸钠水溶液（会产生红棕色）后，于520 nm处记录峰面积。发现浓度与面积之间在10至60 ng/斑点范围内呈线性关系。该方法针对精密度（日间和日内）、重复性和准确性进行了验证。康丝碱的平均回收率为98.34 - 100.25%。该方法用于定量止泻木中的康丝碱。所提出的HPTLC方法被发现是精确、特异、灵敏且准确的，可用于止泻木的常规分析。