Yi Zhong, Zhang Hai-cheng, Zhang Ping, Liu Gang, Lu Peng, Sun Jian-ling, Liu Bin, Guo Ji-hong
Department of Cardiology, People's Hospital, Peking University, Beijing 100044, China.
Zhonghua Yi Xue Za Zhi. 2007 Dec 25;87(48):3433-5.
To test the hypothesis that regional sympathetic innervation in the atria and pulmonary veins are correlated with atrial fibrillation (AF).
Sixteen adult mongrel dogs underwent thoracotomy under general anesthesia. Bilateral cervical vagal trunks were decentralized. Multipolar catheters were placed into right atrial appendage (RAA), left atrial appendage (LAA), left atrium (LA), left superior pulmonary vein (LSPV), left inferior pulmonary vein (LIPV), right superior pulmonary vein (RSPV), and left inferior pulmonary vein (LIPV). The bilateral sympathovagal trunks were stimulated, S1S1 burst stimulation and S1S2 stimulation procedure were performed on different points of RAA, LAA, LA, LSPV, LIPV, RSPV, and LIPV. The TF thus induced was monitored. After that, the dogs were killed with their hearts and lungs were taken out. Immunocytochemical staining of cardiac nerves was performed using anti-tyrosine hydroxylase (TH) antibodies. The nerve fiber density was counted manually for each case and expressed as the mean number per slice.
Two dogs died during the experiment and the whole procedure was completed on 14 dogs. There was no significant difference in the AF induction rate among the most points, however, the AF induction rate of the RIPV was significantly lower than those of the other points (all P < 0.05). The levels of density of TH-positive nerves in the atria and atrial appendages were significantly higher than those in the pulmonary veins (P = 0.02). The density of TH-positive nerves in the dogs with AF was significantly higher than that in the dog without AF (P < 0.05). The innervation of sympathetic nerves in atria and pulmonary veins was highly correlated to the induction of atrial fibrillation (r = 0.83).
Regional sympathetic hyperinnervation plays an important role in atrial fibrillation induction.
检验心房和肺静脉局部交感神经支配与心房颤动（房颤）相关的假设。
16只成年杂种犬在全身麻醉下接受开胸手术。双侧颈迷走神经干去传入化。将多极导管置入右心耳（RAA）、左心耳（LAA）、左心房（LA）、左上肺静脉（LSPV）、左下肺静脉（LIPV）、右上肺静脉（RSPV）和右下肺静脉（LIPV）。刺激双侧交感迷走神经干，在RAA、LAA、LA、LSPV、LIPV、RSPV和LIPV的不同部位进行S1S1串刺激和S1S2刺激程序。监测由此诱发的房颤。之后，处死犬只，取出心脏和肺脏。使用抗酪氨酸羟化酶（TH）抗体对心脏神经进行免疫细胞化学染色。对每例手动计数神经纤维密度，并表示为每切片的平均数。
实验过程中有2只犬死亡，14只犬完成了整个实验。大多数部位的房颤诱发率无显著差异，然而，右下肺静脉的房颤诱发率显著低于其他部位（均P<0.05）。心房和心耳中TH阳性神经的密度水平显著高于肺静脉（P = 0.02）。有房颤的犬中TH阳性神经的密度显著高于无房颤的犬（P<0.05）。心房和肺静脉中交感神经的支配与房颤的诱发高度相关（r = 0.83）。
局部交感神经支配过度在房颤诱发中起重要作用。
