[微波辐射对鼻咽癌细胞丙二醛水平及超氧化物歧化酶活性的影响]

[The effect of microwave radiation on the levels of MDA and the activity of SOD of nasopharyngeal carcinoma cells].

作者信息

Ma Jing, Liu Bin

机构信息

Department of Otorhinolaryngology, Lanzhou Generation Hospital, Lanzhou Command, Lanzhou, 730050, China.

出版信息

Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2008 Feb;22(4):160-2.

PMID:18476619

Abstract

OBJECTIVE

To study the effect of microwave radiation on the levels of malondialdehyde (MDIA) and activity of superoxide dismutase (SOD) of Nasopharyngeal carcinoma cells.

METHOD

The experiment were divided into four groups included control, 10, 20 and 30 mW/cm2 groups. The methods, such as TBA colorimetric assay and modified NBT hydroxylamine method, were used to detect the effect of microwave radiation on the levels of MDA and activity of SOD of nasopharyngeal carcinoma cells.

RESULT

With increase of microwave radiation, TBA assay showed that the levels of MAD were 1.4 and 3.5 times in 20 mW/cm2 group and 30 mW/cm2 group respectively compared with control group, there were significant differences among them (P < 0.05). NBT method shows that the activity of SOD were 68.2%, 46.2% and 36.0% of 10, 20 and 30 mW/cm2 groups respectively compared with control group, there were significant differences among them too (P < 0.01).

CONCLUSION

Microwave Radiation could up-regulate the levels of MAD) while down-regulate the SOD activity.

摘要

目的

研究微波辐射对鼻咽癌细胞丙二醛（MDA）水平和超氧化物歧化酶（SOD）活性的影响。

方法

实验分为四组，包括对照组、10、20和30 mW/cm²组。采用TBA比色法和改良NBT羟胺法等方法检测微波辐射对鼻咽癌细胞MDA水平和SOD活性的影响。

结果

随着微波辐射强度增加，TBA检测显示，20 mW/cm²组和30 mW/cm²组的MDA水平分别是对照组的1.4倍和3.5倍，各组间差异有统计学意义（P<0.05）。NBT法显示，10、20和30 mW/cm²组的SOD活性分别是对照组的68.2%、46.2%和36.0%，各组间差异也有统计学意义（P<0.01）。