Jedlicková Lenka, Gadas David, Havlová Pavla, Havel Josef
Department of Chemistry, Masaryk University, Brno, Czech Republic.
J Agric Food Chem. 2008 Jun 11;56(11):4092-5. doi: 10.1021/jf703675q. Epub 2008 May 14.
Ergosterol is considered to be a suitable indicator of mold infestation in barley and malt. In this study ergosterol levels in different varieties of barley and malt produced in the Czech Republic were determined. A modified high-performance liquid chromatography (HPLC) method was statistically processed, validated (Effivalidation program), and applied to 124 samples of barley and malt. Ergosterol was isolated by extraction and saponification, and the quantification was performed using HPLC with diode array detection. The content of ergosterol ranged between the limit of detection (LOD) and 36.3 mg/kg in barley and between the LOD and 131.1 mg/kg in malt. Ergosterol is presumably connected with metabolites generated when barley grain is attacked by pathogens, and such barley often shows a high overfoaming (gushing) value. However, it was found that the content of ergosterol does not correlate with the degree of beer gushing.
麦角固醇被认为是大麦和麦芽中霉菌侵染的合适指标。在本研究中，测定了捷克共和国生产的不同品种大麦和麦芽中的麦角固醇水平。一种改进的高效液相色谱（HPLC）方法经过统计处理、验证（Effivalidation程序），并应用于124个大麦和麦芽样品。通过萃取和皂化分离麦角固醇，并使用配有二极管阵列检测的HPLC进行定量。大麦中麦角固醇的含量在检测限（LOD）至36.3 mg/kg之间，麦芽中则在LOD至131.1 mg/kg之间。麦角固醇可能与大麦籽粒受到病原体侵袭时产生的代谢产物有关，而这种大麦通常具有较高的溢泡（喷涌）值。然而，发现麦角固醇的含量与啤酒喷涌程度无关。
