成年绒山羊皮肤干细胞的分离与鉴定
Isolation and identification of stem cells from adult cashmere goat skin.
作者信息
Liu Yingchun, Zhou Huanmin, Gao Feng
机构信息
College of Bioengineering and College of Animal Science and Animal Medicine, Inner Mongolia Agricultural University, Huhhot, China.
出版信息
Int J Dermatol. 2008 Jun;47(6):551-6. doi: 10.1111/j.1365-4632.2008.03647.x.
BACKGROUND
Continuously renewing epithelia are maintained by stem cells that slowly proliferate and remain in the tissues for life. It has been known for decades that mouse epithelial stem cells can be selected by adherence to specific integrins.
METHODS
The adherence of cashmere goat epidermal cells to collagen type IV for 10 min was used to obtain enriched epidermal stem cells. The characteristics of the rapidly adherent epidermal cells were determined.
RESULTS
The rapidly adherent epidermal cells exhibited the stem cell characteristics of immaturity, were quiescent, showed a high colony formation efficiency, and expressed candidate surface markers for epidermal stem cells (keratin 15, keratin 19, p63, CD34, and beta1-integrin).
CONCLUSIONS
The rapidly adherent epidermal cells represented the epidermal stem cell population.
背景
持续更新的上皮组织由缓慢增殖并终生留存于组织中的干细胞维持。几十年来已知小鼠上皮干细胞可通过对特定整合素的黏附来筛选。
方法
利用绒山羊表皮细胞与IV型胶原黏附10分钟来获取富集的表皮干细胞。确定快速黏附的表皮细胞的特征。
结果
快速黏附的表皮细胞表现出不成熟、静止的干细胞特征，具有高集落形成效率，并表达表皮干细胞的候选表面标志物（角蛋白15、角蛋白19、p63、CD34和β1整合素）。
结论
快速黏附的表皮细胞代表表皮干细胞群体。
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