Bovera F, Calabrò S, Cutrignelli M I, Infascelli F, Piccolo G, Nizza S, Tudisco R, Nizza A
Dipartimento di Scienze Zootecniche e Ispezione degli Alimenti, Faculty of Veterinary Medicine, University of Napoli Federico II, Naples, Italy.
J Anim Physiol Anim Nutr (Berl). 2008 Jun;92(3):260-71. doi: 10.1111/j.1439-0396.2007.00748.x.
To find equations able to estimate the fermentation characteristics of the caecum from that of faeces, caecal content and faeces of 10 hybrid Hyla rabbits were used as inocula for an in vitro gas production trial. About 1 g of 12 roughages, 11 hays (ryegrass, alfalfa, sulla, oat, vetch, sulla-lolium, vetch-oat, sulla-oat, clover, ryegrass-clover, sulla-vetch-oat) and a wheat straw, was weighed, in triplicate per inoculum, in 120-ml flasks; 75 ml of anaerobic medium and 4 ml of reducing solution were added and the flasks were placed at 39 degrees C. Caecal content and faeces were diluted respectively 1:2 (CI) and 1:8 (FI) with anaerobic medium and were introduced into their respective flasks (10 ml). Gas production was recorded 20 times at 2-24 h intervals throughout fermentation (120 h). The fermentation characteristics (i.e. degraded organic matter, OMd; potential gas production, A; maximum fermentation rate, R(max); volatile fatty acid, VFA; ammonia, NH(3)) were studied by inocula and substrates. The two inocula did not differ in OMd but CI produced significantly higher gas (A, 213.1 vs. 199.4 ml/g, respectively, for CI and FI, p < 0.01) in less time (R(max), 3.08 vs. 2.24 ml/h, respectively, for CI and FI, p < 0.01). CI also produced higher levels of total VFA (57.86 vs. 46.70 mmol/g OM, respectively, for CI and FI, p < 0.01) and showed a higher branched chain proportion (0.023 vs. 0.018, respectively, for CI and FI, p < 0.01). For some parameters (as OMd pH and propionate) the equations for the estimation of caecal fermentation characteristics from that of faeces were accurate (R(2) > 0.8828) and reliable (CV < 10.78%) suggesting that faeces can be successfully used for the estimation of these parameters.
为了找到能够根据粪便发酵特性估算盲肠发酵特性的方程，使用10只杂种海拉兔的盲肠内容物和粪便作为接种物进行体外产气试验。将约1克12种粗饲料、11种草干草（黑麦草、苜蓿、 sulla、燕麦、巢菜、sulla-黑麦草、巢菜-燕麦、sulla-燕麦、三叶草、黑麦草-三叶草、sulla-巢菜-燕麦）和1种小麦秸秆称重，每种接种物重复3次，放入120毫升烧瓶中；加入75毫升厌氧培养基和4毫升还原溶液，将烧瓶置于39℃。盲肠内容物和粪便分别用厌氧培养基按1:2（CI）和1:8（FI）稀释，并分别加入各自的烧瓶中（10毫升）。在整个发酵过程（120小时）中，每隔2 - 24小时记录20次产气情况。通过接种物和底物研究发酵特性（即降解有机物，OMd；潜在产气量，A；最大发酵速率，R(max)；挥发性脂肪酸，VFA；氨，NH(3)）。两种接种物在OMd方面没有差异，但CI在更短的时间内产生了显著更高的气体（A，CI和FI分别为213.1毫升/克和199.4毫升/克，p < 0.０1）（R(max)，CI和FI分别为3.08毫升/小时和2.24毫升/小时，p < 0.０1）。CI还产生了更高水平的总VFA（CI和FI分别为57.86毫摩尔/克OM和46.70毫摩尔/克OM，p < 0.０1），并且显示出更高的支链比例（CI和FI分别为0.023和0.018，p < 0.０1）。对于一些参数（如OMd、pH和丙酸盐），根据粪便发酵特性估算盲肠发酵特性的方程是准确的（R(2) > 0.8828）且可靠的（CV < 10.78%），这表明粪便可以成功用于估算这些参数。
