Robbins B L, Foster J D, Nordlie R C
Department of Biochemistry and Molecular Biology, University of North Dakota, School of Medicine, Grand Forks 58202.
Life Sci. 1991;48(11):1075-81. doi: 10.1016/0024-3205(91)90509-a.
Twenty-five metabolites of glucose, gluconeogenic substrates, and related compounds were examined as potential inhibitors of glucose-6-phosphatase (EC 3.1.3.9) catalytic unit and substrate transport function, using disrupted and intact rat liver microsomes. Inhibitions (competitive) were noted with six. Calculated per cent inhibitions with presumed near-physiologic concentrations of inhibitor and substrate were small. However, when hepatic fructose-1-P concentration is elevated in response to a fructose load, inhibition of glucose-6-phosphatase by fructose-1-P may play a regulatory role, along with fructose-1-P-associated deinhibition of glucokinase, by directing glucose-6-P away from glucose formation and towards glycogen synthesis and glycolysis.
利用破碎的和完整的大鼠肝脏微粒体，检测了25种葡萄糖代谢物、糖异生底物及相关化合物作为葡萄糖-6-磷酸酶（EC 3.1.3.9）催化单位和底物转运功能潜在抑制剂的情况。发现其中六种具有抑制作用（竞争性）。以推测接近生理浓度的抑制剂和底物计算，抑制百分比很小。然而，当肝脏中1-磷酸果糖浓度因果糖负荷而升高时，1-磷酸果糖对葡萄糖-6-磷酸酶的抑制作用可能发挥调节作用，与1-磷酸果糖相关的葡萄糖激酶去抑制作用一起，通过引导6-磷酸葡萄糖远离葡萄糖生成，转向糖原合成和糖酵解。
