Mann A M, Stevenson M, Masui T, Borgeson C D, Cohen S M
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68198.
Oncogene Res. 1991;6(1):65-72.
Malignant transformation of cells in vitro requires the action of cooperating oncogenes. However, the action of a single activated oncogene, if placed under a strong constitutive promoter, is sufficient to transform in vitro established cells. We have investigated the role of cooperating oncogenes in the transformation of normal rat bladder epithelial cells. Effects of polyoma middle T, v-Ha-ras or activated rat c-Ha-ras, independently or in combination with v-myc on in vitro and in vivo behavior of rat bladder epithelial cells were studied. Introduction of polyoma middle T alone, c-Ha-ras or in some cases v-myc into "normal" rat bladder epithelial cells was sufficient for full malignant transformation of these cells. In addition, introduction of polyoma middle T, activated c-Ha-ras or v-myc transformed cells into nude mice, resulted in development of highly invasive adenocarcinomas. These results indicate that full malignant transformation of normal rat bladder epithelial cells did not require the action of introduced cooperating oncogenes.
体外细胞的恶性转化需要协同癌基因的作用。然而，如果将单个激活的癌基因置于强组成型启动子的控制下，其作用足以转化体外已建立的细胞。我们研究了协同癌基因在正常大鼠膀胱上皮细胞转化中的作用。研究了多瘤病毒中T抗原、v-Ha-ras或激活的大鼠c-Ha-ras单独或与v-myc联合对大鼠膀胱上皮细胞体外和体内行为的影响。单独导入多瘤病毒中T抗原、c-Ha-ras或在某些情况下导入v-myc到“正常”大鼠膀胱上皮细胞中，足以使这些细胞完全恶性转化。此外，将多瘤病毒中T抗原、激活的c-Ha-ras或v-myc转化的细胞导入裸鼠体内，会导致高侵袭性腺癌的发生。这些结果表明，正常大鼠膀胱上皮细胞的完全恶性转化并不需要导入的协同癌基因的作用。
