成熟人类精子细胞核中核组蛋白和核精蛋白成分的表征
Characterization of nucleohistone and nucleoprotamine components in the mature human sperm nucleus.
作者信息
Li Yan, Lalancette Claudia, Miller David, Krawetz Stephen A
机构信息
Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Wayne, MI 48201, USA.
出版信息
Asian J Androl. 2008 Jul;10(4):535-41. doi: 10.1111/j.1745-7262.2008.00410.x.
AIM
To simultaneously determine the localization of histones and protamines within human sperm nuclei.
METHODS
Immunofluorescence of the core histones and protamines and fluorescence in situ hybridization of the telomere region of chromosome 16 was assessed in decondensed human sperm nuclei.
RESULTS
Immunofluorescent localization of histones, protamine 1 (PRM1) and protamine 2 (PRM2) along with fluorescence in situ hybridization localization of chromosome 16 telomeric sequences revealed a discrete distribution in sperm nuclei. Histones localized to the posterior ring region (i.e. the sperm nuclear annulus), whereas PRM1 and PRM2 appeared to be dispersed throughout the entire nucleus.
CONCLUSION
The co-localization of the human core sperm histones with the telomeric regions of chromosome 16 is consistent with the reorganization of specific non-protamine regions into a less compacted state.
目的
同时确定组蛋白和鱼精蛋白在人类精子细胞核内的定位。
方法
在解聚的人类精子细胞核中评估核心组蛋白和鱼精蛋白的免疫荧光以及16号染色体端粒区域的荧光原位杂交。
结果
组蛋白、鱼精蛋白1（PRM1）和鱼精蛋白2（PRM2）的免疫荧光定位以及16号染色体端粒序列的荧光原位杂交定位显示在精子细胞核中呈离散分布。组蛋白定位于后环区域（即精子核环），而PRM1和PRM2似乎分散在整个细胞核中。
结论
人类核心精子组蛋白与16号染色体端粒区域的共定位与特定非鱼精蛋白区域重组为较松散状态一致。
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