通过原位杂交将人类鱼精蛋白基因PRM1和PRM2定位于16p13.3。

Chromosomal localization of the human protamine genes, PRM1 and PRM2, to 16p13.3 by in situ hybridization.

作者信息

Viguié F, Domenjoud L, Rousseau-Merck M F, Dadoune J P, Chevaillier P

机构信息

Groupe d'Etrude de la Formation et de la Maturation du Gamète Mâle, Université Paris V, France.

出版信息

Hum Genet. 1990 Jul;85(2):171-4. doi: 10.1007/BF00193191.

DOI:10.1007/BF00193191

PMID:2370044

Abstract

Protamines are sperm-specific proteins that replace histones in the nuclear chromatin of mature spermatozoa. A chromosomal localization of the genes coding for human protamines has been achieved by in situ hybridization. Two cDNA probes of 423 bp and 397 bp containing the entire coding sequence for human protamine 1 (HP1) and human protamine 2 (HP2), respectively, have been used. The genes, called PRM1 and PRM2, have been found, tightly linked, on band 16p13.3. Arguments are given for the existence of these two genes as single copies, PRM1 coding for the unique HP1 protamine and PRM2 coding for a precursor of several proteins belonging to the HP2 family.

摘要

鱼精蛋白是精子特异性蛋白，可取代成熟精子核染色质中的组蛋白。通过原位杂交已实现了对编码人鱼精蛋白的基因进行染色体定位。分别使用了两个包含人鱼精蛋白1（HP1）和人鱼精蛋白2（HP2）完整编码序列的423 bp和397 bp的cDNA探针。已发现这两个基因，称为PRM1和PRM2，紧密连锁于16p13.3带。文中给出了这两个基因为单拷贝存在的证据，PRM1编码独特的HP1鱼精蛋白，PRM2编码属于HP2家族的几种蛋白质的前体。