Uribe Paulina, Fuentes Daniela, Valdés Jorge, Shmaryahu Amir, Zúñiga Alicia, Holmes David, Valenzuela Pablo D T
Fundación Ciencia para la Vida, Av. Zañartu 1482, Nuñoa, Santiago, Chile.
Mar Biotechnol (NY). 2008 Nov-Dec;10(6):692-700. doi: 10.1007/s10126-008-9107-8. Epub 2008 May 14.
Dinoflagellates of the genus Alexandrium are photosynthetic microalgae that have an extreme importance due to the impact of some toxic species on shellfish aquaculture industry. Alexandrium catenella is the species responsible for the production of paralytic shellfish poisoning in Chile and other geographical areas. We have constructed a cDNA library from midexponential cells of A. catenella grown in culture free of associated bacteria and sequenced 10,850 expressed sequence tags (ESTs) that were assembled into 1,021 contigs and 5,475 singletons for a total of 6,496 unigenes. Approximately 41.6% of the unigenes showed similarity to genes with predicted function. A significant number of unigenes showed similarity with genes from other dinoflagellates, plants, and other protists. Among the identified genes, the most expressed correspond to those coding for proteins of luminescence, carbohydrate metabolism, and photosynthesis. The sequences of 9,847 ESTs have been deposited in Gene Bank (accession numbers EX 454357-464203).
亚历山大藻属的甲藻是光合微藻，由于一些有毒物种对贝类养殖业的影响，它们具有极其重要的意义。链状亚历山大藻是导致智利和其他地理区域发生麻痹性贝类中毒的物种。我们从在无相关细菌的培养基中生长的链状亚历山大藻指数中期细胞构建了一个cDNA文库，并对10,850个表达序列标签（EST）进行了测序，这些EST被组装成1,021个重叠群和5,475个单拷贝，总共6,496个单基因。大约41.6%的单基因与具有预测功能的基因显示出相似性。大量单基因与其他甲藻、植物和其他原生生物的基因显示出相似性。在已鉴定的基因中，表达量最高的对应于那些编码发光蛋白、碳水化合物代谢和光合作用的基因。9,847个EST的序列已存入基因库（登录号EX 454357 - 464203）。
