Ward Tiffany M, Edwards Ross A, Tanner Robert D
Department of Chemical Engineering, Vanderbilt University, Nashville, TN 37235, USA.
Appl Biochem Biotechnol. 2007 Apr;137-140(1-12):927-34. doi: 10.1007/s12010-007-9108-5.
A mixture created by blending with a spatula, an egg yolk and an egg white from the same egg can serve as a binary system for testing to see how well foam fractionation can be used to separate two different groups of proteins naturally found together. This mixture of two phases is particularly attractive for such a study because the two phases can be visualized distinctly when in their separated states. It has been shown that air alone at a low flow rate and with little or no water added can effect visually clean separations of egg yolk from egg white, making this a "green" separation process. The white precedes the yolk in the process, which takes less than 10 min at a laboratory scale.
用刮刀将同一个鸡蛋的蛋黄和蛋清混合而成的混合物,可作为一个二元体系,用于测试泡沫分离法在分离天然共存的两组不同蛋白质方面的效果。这种两相混合物对于此类研究特别有吸引力,因为在两相分离状态下可以清晰地观察到它们。研究表明,仅以低流速通入空气且几乎不加水或不加水时,就能实现蛋黄与蛋清在视觉上的清晰分离,这使得该过程成为一种“绿色”分离方法。在实验室规模下,分离过程中蛋清先于蛋黄出现,耗时不到10分钟。