Murakami M, Sakai H, Kodama A, Mori T, Maruo K, Yanai T, Masegi T
Laboratory of Veterinary Pathology, Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
J Comp Pathol. 2008 Jul;139(1):1-7. doi: 10.1016/j.jcpa.2008.02.001. Epub 2008 May 13.
To investigate whether anti-apoptotic factors play a role in the malignant growth of canine haemangiosarcomas (HSAs), 83 HSAs and 22 haemangiomas were examined immunohistochemically for bcl-2 and survivin expression. Additionally, bcl-2 and survivin mRNA expression was quantified by semiquantitative real-time reverse transcription-polymerase chain reaction (RT-PCR). Immunolabelling for bcl-2 was observed in 50 of the 83 HSA samples (60.2%) but in none of the haemangiomas. The average survivin positive index was 24.7% in the HSAs and 0.6% in the haemangiomas. In contrast to the high average value for survivin mRNA expression, which was approximately six times that for the haemangiomas, no significant difference was observed between HSAs and haemangiomas for the average bcl-2 mRNA expression level. The discrepancy between bcl-2 mRNA and bcl-2 protein expression requires further investigation, but the results suggest that malignant proliferation in canine HSAs is associated with bcl-2 and survivin expression.
为了研究抗凋亡因子是否在犬血管肉瘤（HSAs）的恶性生长中发挥作用，对83例HSAs和22例血管瘤进行免疫组织化学检测，以观察bcl-2和生存素的表达情况。此外，通过半定量实时逆转录-聚合酶链反应（RT-PCR）对bcl-2和生存素mRNA的表达进行定量分析。在83例HSA样本中，有50例（60.2%）观察到bcl-2免疫染色阳性，而血管瘤样本中均未观察到。HSAs中生存素的平均阳性指数为24.7%，血管瘤中为0.6%。与血管瘤相比，生存素mRNA表达的平均值较高，约为血管瘤的6倍，但HSAs和血管瘤之间的平均bcl-2 mRNA表达水平未观察到显著差异。bcl-2 mRNA与bcl-2蛋白表达之间的差异需要进一步研究，但结果表明犬HSAs的恶性增殖与bcl-2和生存素的表达有关。
