Clark L, Lange A B, Zhang J R, Tobe S S
Department of Biology, University of Toronto Mississauga, Mississauga, Ont., Canada L5L 1C6.
J Insect Physiol. 2008 Jun;54(6):949-58. doi: 10.1016/j.jinsphys.2008.03.007. Epub 2008 Mar 25.
Dippu-allatostatins (ASTs) have pleiotropic effects in Locusta migratoria. Dippu-ASTs act as releasing factors for adipokinetic hormone I (AKH I) from the corpus cardiacum (CC) and also alter juvenile hormone (JH) biosynthesis and release from the corpus allatum (CA). Dippu-AST-like immunoreactivity is found within lateral neurosecretory cells (LNCs) of the brain and axons within the paired nervi corporis cardiaci II (NCC II) to the CC and the CA, where there are extensive processes and nerve endings over both of these neuroendocrine organs. There was co-localization of Dippu-AST-like and proctolin-like immunoreactivity within these regions. Dippu-ASTs increase the release of AKH I in a dose-dependent manner, with thresholds below 10(-11)M (Dippu-AST 7) and between 10(-13) and 10(-12)M (Dippu-AST 2). Both proctolin and Dippu-AST 2 caused an increase in the cAMP content of the glandular lobe of the CC. Dippu-AST 2 also altered the release of JH from the locust CA, but this effect depended on the concentration of peptide and the basal release rates of the CA. These physiological effects for Dippu-ASTs in Locusta have not been shown previously.
地普-咽侧体抑制素（ASTs）在飞蝗中有多种效应。地普-ASTs作为促动激素I（AKH I）从心侧体（CC）释放的因子，同时也改变保幼激素（JH）的生物合成及从咽侧体（CA）的释放。在地普-AST样免疫反应性存在于脑的侧神经分泌细胞（LNCs）以及成对的心侧体神经II（NCC II）内通向CC和CA的轴突中，在这两个神经内分泌器官上均有广泛的突起和神经末梢。在这些区域存在地普-AST样和促肠肌肽样免疫反应性的共定位。地普-ASTs以剂量依赖的方式增加AKH I的释放，阈值低于10^(-11)M（地普-AST 7）以及在10^(-13)至10^(-12)M之间（地普-AST 2）。促肠肌肽和地普-AST 2均导致CC腺叶的cAMP含量增加。地普-AST 2也改变了飞蝗CA中JH的释放，但这种效应取决于肽的浓度和CA的基础释放速率。地普-ASTs在飞蝗中的这些生理效应此前尚未见报道。
