Dhut S, Gibbons B, Chaplin T, Young B D
ICRF Medical Oncology Laboratory, St. Bartholomew's Hospital, London, UK.
Leukemia. 1991 Jan;5(1):49-55.
A lymphoblastoid cell line (SD-1) has been established by Epstein-Barr virus immortalisation of Philadelphia chromosome positive acute lymphoblastic leukaemia (ALL) cells. Using newly derived anti-bcr monoclonal and anti-abl polyclonal antibodies it was demonstrated that both the original leukaemic cells and the derived cell line expressed the p190 form of the bcr-abl protein found in a proportion of cases of Philadelphia chromosome positive ALL. Interestingly, the leukaemia and the derived cell line each displayed different, clonal patterns of immunoglobulin gene rearrangements providing direct evidence that the t(9;22) translocation which results in the expression of the p190 bcr-abl protein must occur before immunoglobulin heavy chain gene rearrangement. In contrast to the leukaemia, which had multiple chromosome abnormalities in addition to the t(9;22), the cell line had the t(9;22) translocation as its sole abnormality. Although SD-1 cells were demonstrated to express continuously the p190 bcr-abl protein, they were unable to form colonies in soft agar and did not cause tumours in splenectomised nude mice. This cell line therefore represents an appropriate target cell line in which to examine the cooperativity of the p190 bcr-abl protein with other activated oncogene products.
通过爱泼斯坦-巴尔病毒永生化费城染色体阳性急性淋巴细胞白血病（ALL）细胞，建立了一个淋巴母细胞样细胞系（SD-1）。使用新获得的抗bcr单克隆抗体和抗abl多克隆抗体，证实原始白血病细胞和衍生细胞系均表达了在一部分费城染色体阳性ALL病例中发现的p190形式的bcr-abl蛋白。有趣的是，白血病细胞和衍生细胞系各自显示出不同的免疫球蛋白基因重排克隆模式，这直接证明导致p190 bcr-abl蛋白表达的t(9;22)易位必定发生在免疫球蛋白重链基因重排之前。与除t(9;22)外还有多种染色体异常的白血病不同，该细胞系仅存在t(9;22)易位这一异常。尽管已证明SD-1细胞持续表达p190 bcr-abl蛋白，但它们无法在软琼脂中形成集落，也不会在脾切除的裸鼠中引发肿瘤。因此，该细胞系代表了一个合适的靶细胞系，可用于研究p190 bcr-abl蛋白与其他活化癌基因产物的协同作用。
