Ryall B, Davies J C, Wilson R, Shoemark A, Williams H D
Dept of Life Sciences, Division of Biology, Faculty of Natural Sciences, Imperial College London, Sir Alexander Fleming Building, London, SW7 2AZ, UK.
Eur Respir J. 2008 Sep;32(3):740-7. doi: 10.1183/09031936.00159607. Epub 2008 May 14.
In patients with cystic fibrosis (CF) and non-CF bronchiectasis, Pseudomonas aeruginosa is the most important respiratory pathogen. It is able to synthesise hydrogen cyanide, a potent inhibitor of cellular respiration. The present study investigated whether cyanide is present in the sputum of CF and non-CF bronchiectasis patients infected with P. aeruginosa, and whether the detection of cyanide affected lung function. Cyanide was measured in sputum using a cyanide ion selective electrode. Cyanide was detected in sputum from 15 out of 25 CF and non-CF bronchiectasis patients with current P. aeruginosa infection; however, it was not detected in any of the 10 patients without this organism. Maximum levels were 130 microM (mean+/-SE 72+/-6.6 microM). Concurrent lung function data were available on all 21 P. aeruginosa-infected CF patients; the group with measurable sputum cyanide (n = 11) was not different from those without (n = 10) on the basis of age or sex. However, those with detectable cyanide had significantly poorer lung function than those without (forced expiratory volume in one second (% predicted) 26.8+/-3.8 versus 46.0+/-6.7%; forced vital capacity (% pred) 44.4+/-4.9 versus 60.1+/-7.7%). Cyanide is detectable in sputum from cystic fibrosis and non-cystic fibrosis bronchiectasis patients infected with Pseudomonas aeruginosa, and is also associated with impaired lung function.
在囊性纤维化（CF）患者和非CF支气管扩张患者中，铜绿假单胞菌是最重要的呼吸道病原体。它能够合成氰化氢，一种细胞呼吸的强效抑制剂。本研究调查了感染铜绿假单胞菌的CF和非CF支气管扩张患者的痰液中是否存在氰化物，以及氰化物的检测是否会影响肺功能。使用氰离子选择性电极测量痰液中的氰化物。在25例当前感染铜绿假单胞菌的CF和非CF支气管扩张患者中，有15例痰液中检测到氰化物；然而，在10例未感染该菌的患者中均未检测到。最高水平为130微摩尔/升（均值±标准误72±6.6微摩尔/升）。所有21例感染铜绿假单胞菌的CF患者均有同时期的肺功能数据；有可测量痰液氰化物的组（n = 11）在年龄或性别方面与无氰化物的组（n = 10）无差异。然而，可检测到氰化物的患者肺功能明显比未检测到的患者差（一秒用力呼气量（预测值%）26.8±3.8对46.0±6.7%；用力肺活量（预测值%）44.4±4.9对60.1±7.7%）。在感染铜绿假单胞菌的囊性纤维化和非囊性纤维化支气管扩张患者的痰液中可检测到氰化物，并且还与肺功能受损有关。
