Wada E, Way J, Shapira H, Kusano K, Lebacq-Verheyden A M, Coy D, Jensen R, Battery J
Laboratory of Neurochemistry, National Institute of Neurological Disorders and Stroke, National Institutes of Health Bethesda, Maryland 20892.
Neuron. 1991 Mar;6(3):421-30. doi: 10.1016/0896-6273(91)90250-4.
Recent binding studies in the central nervous system and other tissues provide evidence that the mammalian bombesin-like peptides, gastrin-releasing peptide (GRP) and neuromedin-B (NMB), exert their numerous physiological effects through at least two different receptors. We describe the structure and expression of a cloned NMB-preferring bombesin receptor (NMB-R) with properties distinct from a GRP-preferring bombesin receptor (GRP-R) reported previously. In particular, the NMB-R shows higher affinity binding to NMB than to GRP in BALB 3T3 fibroblasts expressing the cloned NMB-R. The distinct regional distribution of NMB-R and GRP-R mRNA in the brain suggests that both bombesin receptor subtypes play independent roles in mediating many of the dramatic effects of bombesin-like peptides in the central nervous system.
最近在中枢神经系统和其他组织中的结合研究表明，哺乳动物的铃蟾肽样肽，即胃泌素释放肽（GRP）和神经介素B（NMB），至少通过两种不同的受体发挥其多种生理作用。我们描述了一种克隆的NMB偏好性铃蟾肽受体（NMB-R）的结构和表达，其特性与先前报道的GRP偏好性铃蟾肽受体（GRP-R）不同。特别是，在表达克隆的NMB-R的BALB 3T3成纤维细胞中，NMB-R对NMB的亲和力高于对GRP的亲和力。NMB-R和GRP-R mRNA在脑中的不同区域分布表明，两种铃蟾肽受体亚型在介导中枢神经系统中铃蟾肽样肽的许多显著作用中发挥独立作用。
