通过定量检测和表面增强激光解吸电离飞行时间质谱分析对LNCap细胞和DU145细胞进行蛋白质组学比较研究。
A comparative study on proteomics between LNCap and DU145 cells by quantitative detection and SELDI analysis.
作者信息
Sun Weigui, Ye Zhangqun, Mi Zhenguo, Shi Tianliang, Han Cunzhi, Guo Sutang
机构信息
Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
出版信息
J Huazhong Univ Sci Technolog Med Sci. 2008 Apr;28(2):174-8. doi: 10.1007/s11596-008-0215-5. Epub 2008 May 15.
The differences in intracellular and extracellular protein expressions between human prostate cancer lines LNCap and DU145 were examined. The proteins of the two cell lines were extracted and condensed by using protein extraction kits. And the intracellular and extracellular proteins were quantitatively detected on a micro-plate reader by using bicinchoninic acid (BCA) method. The proteins in cell culture fluid were qualitatively assayed by SELDI-TOF-MS. The results showed that the intracellular protein contents of LNCap cells were extremely higher than those of DU145 cells. After serum-free culture, both intracellular and extracellular protein contents of LNCap and DU145 were decreased to some extent. And the intracellular proteins were decreased by 5% in LNCap and by 36% in DU145 respectively, while the extracellular proteins were decreased by 89% in LNCap and 96% in DU145 respectively. SELDI assay revealed that there were 5 marker proteins in LNCap and 6 in DU145. Although both LNCap and DU145 cell lines originated from human prostate cancer, they had some differences in protein expression.
研究了人前列腺癌LNCap和DU145细胞系细胞内和细胞外蛋白质表达的差异。使用蛋白质提取试剂盒提取并浓缩两种细胞系的蛋白质。采用二喹啉甲酸（BCA）法在酶标仪上对细胞内和细胞外蛋白质进行定量检测。用表面增强激光解吸电离飞行时间质谱（SELDI-TOF-MS）对细胞培养液中的蛋白质进行定性分析。结果显示，LNCap细胞的细胞内蛋白质含量极高于DU145细胞。无血清培养后，LNCap和DU145细胞内和细胞外蛋白质含量均有一定程度下降。其中，LNCap细胞内蛋白质分别下降了5%，DU145细胞内蛋白质下降了36%；LNCap细胞外蛋白质下降了89%，DU145细胞外蛋白质下降了96%。SELDI分析显示，LNCap有5种标志物蛋白，DU145有6种。虽然LNCap和DU145细胞系均源自人前列腺癌，但它们在蛋白质表达上存在一些差异。
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