亚黄盖鹅膏菌的细胞毒性成分。

Cytotoxic constituents of Amanita subjunquillea.

作者信息

Kim Ki Hyun, Choi Sang Un, Park Ki Moon, Seok Soon Ja, Lee Kang Ro

机构信息

Natural Products Laboratory, College of Pharmacy, Sungkyunkwan University, 300 Chonchondong, Jangan-ku, Suwon, 440-746, Korea.

出版信息

Arch Pharm Res. 2008 May;31(5):579-86. doi: 10.1007/s12272-001-1196-3. Epub 2008 May 15.

DOI:10.1007/s12272-001-1196-3

PMID:18481012

Abstract

As part of our systematic study of Korean toxic mushrooms, we have investigated the constituents of Amanita subjunquillea. The column chromatographic separation of the MeOH extract of A. subjunquillea led to the isolation of four ergosterols, two cerebrosides and four cyclopeptides. Their structures were determined by spectroscopic methods to be (22E,24R)-5alpha,8alpha-epidioxyergosta-6,9,22-triene-3beta-ol (1), (22E,24R)-5alpha,8alpha-epidioxyergosta-6,22-dien-3beta-ol (2), (22E,24R)-5alpha,6alpha-epoxyergosta-8,22-diene-3beta,7beta-diol (3), (24S)-ergost-7-en-3beta-ol (4), 8,9-dihydrosoyacerebroside I (5), soyacerebroside I (6), beta-amanitin (7), phalloin (8), alpha-amanitin (9), and phalloidin (10). The compounds 1-6 and 8 were isolated for the first time from this mushroom. The isolated compounds were evaluated for the cytotoxicity against A549, SK-OV-3, SK-MEL-2 and HCT15 cells. Compound 9 exhibited significant cytotoxic activity against A549, SK-OV-3, SK-MEL-2 and HCT15 with ED(50) values of 1.47, 0.26, 1.57 and 1.32 microM, respectively.

摘要

作为我们对韩国有毒蘑菇系统研究的一部分，我们对亚橙盖鹅膏菌的成分进行了研究。亚橙盖鹅膏菌甲醇提取物的柱色谱分离得到了四种麦角甾醇、两种脑苷脂和四种环肽。通过光谱方法确定它们的结构分别为(22E,24R)-5α,8α-环氧麦角甾-6,9,22-三烯-3β-醇(1)、(22E,24R)-5α,8α-环氧麦角甾-6,22-二烯-3β-醇(2)、(22E,24R)-5α,6α-环氧麦角甾-8,22-二烯-3β,7β-二醇(3)、(24S)-麦角甾-7-烯-3β-醇(4)、8,9-二氢大豆脑苷脂I(5)、大豆脑苷脂I(6)、β-鹅膏毒肽(7)、鬼笔菌素(8)、α-鹅膏毒肽(9)和鬼笔环肽(10)。化合物1-6和8首次从该蘑菇中分离得到。对分离得到的化合物进行了针对A549、SK-OV-3、SK-MEL-2和HCT15细胞的细胞毒性评估。化合物9对A549、SK-OV-3、SK-MEL-2和HCT15表现出显著的细胞毒性活性，ED(50)值分别为1.47、0.26、1.57和1.32微摩尔。