燕麦籽粒中单半乳糖基、二半乳糖基、三半乳糖基和四半乳糖基二酰基甘油及其天然内酯的鉴定。
The identification of mono-, di-, tri-, and tetragalactosyl-diacylglycerols and their natural estolides in oat kernels.
作者信息
Moreau Robert A, Doehlert Douglas C, Welti Ruth, Isaac Giorgis, Roth Mary, Tamura Pamela, Nuñez Alberto
机构信息
US Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA 19038, USA.
出版信息
Lipids. 2008 Jun;43(6):533-48. doi: 10.1007/s11745-008-3181-6. Epub 2008 May 15.
Oat kernels were extracted with methanol, and glycolipid-enriched fractions were prepared using silica solid phase extraction. Using direct infusion electrospray ionization (ESI) tandem mass spectrometry (MS), high performance liquid chromatography (HPLC)-ESI-MS, and HPLC-atmospheric pressure chemical ionization (APCI)-MS, we confirmed previous reports that digalactosyldiacylglycerol (DGDG) was the most abundant glycolipid in oat kernels and confirmed a previous report of the presence of a DGDG mono-estolide in oat kernels. In the current study we also identified several additional natural galactolipid estolides: two new DGDG estolides (di- and tri-estolides), two trigalactosyldiacylglycerol (TriGDG) estolides (mono- and di-estolides), and one tetragalactosyldiacylglycerol (TetraGDG) estolide (mono-estolide). The levels of total galactolipid estolides in oat kernels were estimated to be about 29% of the total glycolipid fraction. To our knowledge, this report is the first evidence of natural di- and tri-estolides of polar lipids.
用甲醇提取燕麦籽粒，并用硅胶固相萃取法制备富含糖脂的馏分。使用直接进样电喷雾电离（ESI）串联质谱（MS）、高效液相色谱（HPLC）-ESI-MS和HPLC-大气压化学电离（APCI）-MS，我们证实了之前的报道，即二半乳糖基二酰基甘油（DGDG）是燕麦籽粒中含量最丰富的糖脂，并证实了之前关于燕麦籽粒中存在DGDG单酯化物的报道。在本研究中，我们还鉴定出了几种额外的天然半乳糖脂酯化物：两种新的DGDG酯化物（二酯化物和三酯化物）、两种三半乳糖基二酰基甘油（TriGDG）酯化物（单酯化物和二酯化物）以及一种四半乳糖基二酰基甘油（TetraGDG）酯化物（单酯化物）。燕麦籽粒中总半乳糖脂酯化物的含量估计约占总糖脂馏分的29%。据我们所知，本报告是极性脂质天然二酯化物和三酯化物的首个证据。
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