Yamauchi R, Aizawa K, Inakuma T, Kato K
Department of Bioprocessing, Faculty of Agriculture, Gifu University, 1-1 Yanagido, Gifu City, Gifu 501-1193, Japan.
J Agric Food Chem. 2001 Feb;49(2):622-7. doi: 10.1021/jf001192k.
Five major glycolipid classes (acylated steryl glucoside, steryl glucoside, monogalactosyldiacylglycerol, digalactosyldiacylglycerol, and glucocerebroside) from fruit pastes of red bell pepper were separated by silica gel column chromatography. The molecular species of each glycolipid were separated and characterized by reversed-phase high-performance liquid chromatography coupled with on-line mass spectrometry using atmospheric pressure chemical ionization. The molecular species of steryl glucoside were beta-sitosteryl and campesteryl glucosides, and those of the acylated steryl glucoside were their fatty acid esters. The dilinolenoyl species was predominant in monogalactosyldiacylglycerol in addition to small amounts of another five molecular species, whereas digalactosyldiacylglycerol consisted of seven molecular species varying in their degree of unsaturation. The glucocerebroside class contained at least seven molecular species, which were characterized by proton nuclear magnetic resonance spectroscopy.
采用硅胶柱色谱法分离了红甜椒果泥中的五大类糖脂（酰化甾醇葡萄糖苷、甾醇葡萄糖苷、单半乳糖基二酰基甘油、双半乳糖基二酰基甘油和葡萄糖脑苷脂）。通过反相高效液相色谱结合大气压化学电离在线质谱法对每种糖脂的分子种类进行了分离和表征。甾醇葡萄糖苷的分子种类为β-谷甾醇葡萄糖苷和菜油甾醇葡萄糖苷，酰化甾醇葡萄糖苷的分子种类为它们的脂肪酸酯。除少量其他五种分子种类外，二亚麻酰基种类在单半乳糖基二酰基甘油中占主导地位，而双半乳糖基二酰基甘油由七种不饱和度不同的分子种类组成。葡萄糖脑苷脂类至少包含七种分子种类，通过质子核磁共振光谱对其进行了表征。
