Wang Jia-Lin, Wang Jin-Xing, Zhao Xiao-Fan
School of Life Sciences, Shandong University, Jinan, Shandong, China.
Arch Insect Biochem Physiol. 2008 Jun;68(2):79-88. doi: 10.1002/arch.20250.
Acyl-CoA-binding protein (ACBP), also known as the diazepam-binding inhibitor (DBI), has been identified in diverse species and is evolutionarily conserved in plants and animals. In a recent study, an ACBP cDNA (HaACBP) encoding 85 amino acids was isolated from the cotton bollworm Helicoverpa armigera. The isolated protein is highly homologous to the ACBP present in the Bombyx mori midgut, where it is highly expressed. Northern and Western blot analyses revealed that HaACBP is expressed predominantly in the midgut. Moreover, Northern blotting revealed that HaACBP was probably stimulated by a high juvenile hormone titer at ecdysis and increased along with feeding at 12 h post-ecdysis. Immunohistochemistry of the midgut revealed that HaACBP is localized in columnar cells. Data from the Northern blotting and immunohistochemistry suggested that HaACBP was expressed during the larval period and is probably responsible for nutrition absorption. However, Western blot analysis of the midgut at different developmental stages indicated that HaACBP was upregulated during larval molting and metamorphosis, which suggested that HaACBP expression was posttranscriptionally regulated.
酰基辅酶A结合蛋白（ACBP），也被称为地西泮结合抑制剂（DBI），已在多种物种中被鉴定出来，并且在植物和动物中具有进化保守性。在最近的一项研究中，从棉铃虫Helicoverpa armigera中分离出了一个编码85个氨基酸的ACBP cDNA（HaACBP）。分离出的蛋白质与家蚕中肠中存在的ACBP高度同源，且在家蚕中肠中高表达。Northern印迹和Western印迹分析表明，HaACBP主要在中肠中表达。此外，Northern印迹显示，HaACBP可能在蜕皮时受到高保幼激素滴度的刺激，并在蜕皮后12小时随着进食而增加。中肠的免疫组织化学显示，HaACBP定位于柱状细胞中。Northern印迹和免疫组织化学的数据表明，HaACBP在幼虫期表达，可能负责营养吸收。然而，对不同发育阶段中肠的Western印迹分析表明，HaACBP在幼虫蜕皮和变态期间上调，这表明HaACBP的表达受到转录后调控。
