Gilpin N W, Richardson H N, Lumeng L, Koob G F
Committee on Neurobiology of Addictive Disorders, The Scripps Research Institute, La Jolla, California 92037, USA.
Alcohol Clin Exp Res. 2008 Sep;32(9):1688-96. doi: 10.1111/j.1530-0277.2008.00678.x. Epub 2008 May 14.
Chronic intermittent alcohol vapor exposure and selective breeding procedures have been used separately for many years to model specific aspects of alcohol dependence. The purpose of the present investigation was to combine these 2 approaches by exposing alcohol-preferring (P) rats to chronic intermittent alcohol vapor for extended periods of time and then testing them for operant alcohol responding in parallel with a group of outbred Wistar rats at multiple time points following the termination of vapor exposure.
P rats (n = 20) and Wistar rats (n = 18) were trained to respond for 10% (w/v) ethanol in an operant situation, then divided into groups matched for intake levels. Animals were then exposed to chronic intermittent alcohol vapor (14 hours ON/10 hours OFF) or air for 8 weeks. Rats were then tested for operant alcohol responding under various conditions and at multiple time points during alcohol withdrawal (6 hours) and protracted abstinence (1 to 15 days).
Chronic alcohol vapor exposure produced similar increases in operant alcohol responding in P rats and Wistar rats during acute withdrawal and protracted abstinence.
These results illustrate the separate and combined effects of genetic selection for high alcohol preference and dependence on alcohol drinking behavior. Furthermore, these results confirm past findings that dependent rats consume more alcohol than nondependent controls well into abstinence following extended periods of alcohol vapor exposure.
慢性间歇性酒精蒸汽暴露和选择性育种程序多年来一直被分别用于模拟酒精依赖的特定方面。本研究的目的是将这两种方法结合起来，让偏爱酒精的（P）大鼠长时间暴露于慢性间歇性酒精蒸汽中，然后在蒸汽暴露终止后的多个时间点，与一组远交系Wistar大鼠并行测试它们对酒精的操作性反应。
将P大鼠（n = 20）和Wistar大鼠（n = 18）在操作性条件下训练以对10%（w/v）乙醇做出反应，然后根据摄入量水平分成匹配的组。然后将动物暴露于慢性间歇性酒精蒸汽（开14小时/关10小时）或空气中8周。然后在酒精戒断（6小时）和长期禁欲（1至15天）期间的各种条件下和多个时间点测试大鼠对酒精的操作性反应。
在急性戒断和长期禁欲期间，慢性酒精蒸汽暴露使P大鼠和Wistar大鼠对酒精的操作性反应有相似的增加。
这些结果说明了对高酒精偏爱进行基因选择以及依赖酒精对饮酒行为的单独和联合影响。此外，这些结果证实了过去的研究发现，即经过长时间酒精蒸汽暴露后，依赖酒精的大鼠在禁欲期比非依赖酒精的对照组消耗更多的酒精。
