• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

蛋白磷酸酶2A专门使猴病毒40大T抗原在参与DNA结合活性调节的残基处发生去磷酸化。

Protein phosphatase 2A dephosphorylates simian virus 40 large T antigen specifically at residues involved in regulation of DNA-binding activity.

作者信息

Scheidtmann K H, Virshup D M, Kelly T J

机构信息

Institut für Genetik, Universität Bonn, Federal Republic of Germany.

出版信息

J Virol. 1991 Apr;65(4):2098-101. doi: 10.1128/JVI.65.4.2098-2101.1991.

DOI:10.1128/JVI.65.4.2098-2101.1991
PMID:1848320
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC240073/
Abstract

Treatment of purified simian virus 40 large T antigen (LT) with protein phosphatase 2A stimulates LT-dependent DNA unwinding and replication (D. M. Virshup, M. G. Kauffman, and T. J. Kelly, EMBO J. 8: 3891-3898, 1989). The specificity of the catalytic subunit of protein phosphatase 2A toward LT was investigated by two-dimensional peptide mapping. Increasing amounts of phosphatase sequentially removed the phosphates from serine residues 120, 123, 677, and perhaps 679, residues which have been implicated in regulating the DNA-binding activity of LT.

摘要

用蛋白磷酸酶2A处理纯化的猴病毒40大T抗原（LT）可刺激LT依赖的DNA解旋和复制（D.M.维尔舒普、M.G.考夫曼和T.J.凯利，《欧洲分子生物学组织杂志》8:3891 - 3898，1989年）。通过二维肽图分析研究了蛋白磷酸酶2A催化亚基对LT的特异性。逐渐增加的磷酸酶量依次从丝氨酸残基120、123、677以及可能的679上去除磷酸基团，这些残基与调节LT的DNA结合活性有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/210f/240073/c7103c157bf9/jvirol00047-0444-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/210f/240073/0fa97edaa5e9/jvirol00047-0443-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/210f/240073/c7103c157bf9/jvirol00047-0444-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/210f/240073/0fa97edaa5e9/jvirol00047-0443-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/210f/240073/c7103c157bf9/jvirol00047-0444-a.jpg

相似文献

1
Protein phosphatase 2A dephosphorylates simian virus 40 large T antigen specifically at residues involved in regulation of DNA-binding activity.蛋白磷酸酶2A专门使猴病毒40大T抗原在参与DNA结合活性调节的残基处发生去磷酸化。
J Virol. 1991 Apr;65(4):2098-101. doi: 10.1128/JVI.65.4.2098-2101.1991.
2
Mechanism of activation of simian virus 40 DNA replication by protein phosphatase 2A.蛋白磷酸酶2A激活猿猴病毒40 DNA复制的机制。
Mol Cell Biol. 1992 Nov;12(11):4883-95. doi: 10.1128/mcb.12.11.4883-4895.1992.
3
Selective ability of S-phase cell extracts to dephosphorylate SV40 large T antigen in vitro.S期细胞提取物在体外使SV40大T抗原去磷酸化的选择性能力。
Oncogene. 1992 May;7(5):1011-4.
4
Simian virus 40 large T-antigen-dependent DNA replication is activated by protein phosphatase 2A in vitro.在体外，猿猴病毒40大T抗原依赖性DNA复制被蛋白磷酸酶2A激活。
J Virol. 1990 May;64(5):2380-3. doi: 10.1128/JVI.64.5.2380-2383.1990.
5
Activation of SV40 DNA replication in vitro by cellular protein phosphatase 2A.细胞蛋白磷酸酶2A在体外激活SV40 DNA复制
EMBO J. 1989 Dec 1;8(12):3891-8. doi: 10.1002/j.1460-2075.1989.tb08568.x.
6
Effects of in vitro dephosphorylation on DNA-binding and DNA helicase activities of simian virus 40 large tumor antigen.体外去磷酸化对猴病毒40大肿瘤抗原的DNA结合及DNA解旋酶活性的影响
J Virol. 1988 Apr;62(4):1258-65. doi: 10.1128/JVI.62.4.1258-1265.1988.
7
Different oligomeric forms of protein phosphatase 2A activate and inhibit simian virus 40 DNA replication.蛋白磷酸酶2A的不同寡聚形式激活并抑制猿猴病毒40 DNA复制。
Mol Cell Biol. 1994 Jul;14(7):4616-23. doi: 10.1128/mcb.14.7.4616-4623.1994.
8
Removal of serine phosphates from simian virus 40 large T antigen increases its ability to stimulate DNA replication in vitro but has no effect on ATPase and DNA binding.去除猴病毒40大T抗原上的丝氨酸磷酸会增强其在体外刺激DNA复制的能力，但对ATP酶和DNA结合没有影响。
J Virol. 1987 Nov;61(11):3373-80. doi: 10.1128/JVI.61.11.3373-3380.1987.
9
Dephosphorylation of simian virus 40 large-T antigen and p53 protein by protein phosphatase 2A: inhibition by small-t antigen.蛋白磷酸酶2A对猿猴病毒40大T抗原和p53蛋白的去磷酸化作用：小t抗原的抑制作用
Mol Cell Biol. 1991 Apr;11(4):1996-2003. doi: 10.1128/mcb.11.4.1996-2003.1991.
10
Control of protein phosphatase 2A by simian virus 40 small-t antigen.猿猴病毒40小t抗原对蛋白磷酸酶2A的调控
Mol Cell Biol. 1991 Apr;11(4):1988-95. doi: 10.1128/mcb.11.4.1988-1995.1991.

引用本文的文献

1
Phosphorylation of Human Polyomavirus Large and Small T Antigens: An Ignored Research Field.人多瘤病毒大、小 T 抗原的磷酸化：一个被忽视的研究领域。
Viruses. 2023 Nov 9;15(11):2235. doi: 10.3390/v15112235.
2
Proteomic Analyses of Whitefly-Begomovirus Interactions Reveal the Inhibitory Role of Tumorous Imaginal Discs in Viral Retention.白蛾-烟粉虱病毒互作的蛋白质组学分析揭示了肿瘤性 imaginal discs 在病毒滞留中的抑制作用。
Front Immunol. 2020 Aug 4;11:1596. doi: 10.3389/fimmu.2020.01596. eCollection 2020.
3
Dephosphorylation of JC virus agnoprotein by protein phosphatase 2A: inhibition by small t antigen.

本文引用的文献

1
Differential phosphorylation of cytoplasmic and nuclear variants of simian virus 40 large T antigen encoded by simian virus 40-adenovirus 7 hybrid viruses.猴病毒40-腺病毒7杂交病毒编码的猴病毒40大T抗原细胞质和细胞核变体的差异磷酸化作用
J Virol. 1984 May;50(2):636-40. doi: 10.1128/JVI.50.2.636-640.1984.
2
DNA-binding activity of simian virus 40 large T antigen correlates with a distinct phosphorylation state.猿猴病毒40大T抗原的DNA结合活性与一种独特的磷酸化状态相关。
J Virol. 1984 Apr;50(1):1-12. doi: 10.1128/JVI.50.1.1-12.1984.
3
Metabolic turnover of phosphorylation sites in simian virus 40 large T antigen.
蛋白磷酸酶2A对JC病毒辅助蛋白的去磷酸化作用：受小t抗原抑制。
Virology. 2008 Jun 5;375(2):464-79. doi: 10.1016/j.virol.2008.02.020. Epub 2008 Mar 18.
4
JC virus T' proteins encoded by alternatively spliced early mRNAs enhance T antigen-mediated viral DNA replication in human cells.由可变剪接的早期mRNA编码的JC病毒T'蛋白增强了人细胞中T抗原介导的病毒DNA复制。
J Neurovirol. 2001 Jun;7(3):250-64. doi: 10.1080/13550280152403290.
5
Protein phosphatase 2A: a highly regulated family of serine/threonine phosphatases implicated in cell growth and signalling.蛋白磷酸酶2A：一个与细胞生长和信号传导相关的丝氨酸/苏氨酸磷酸酶的高度调控家族。
Biochem J. 2001 Feb 1;353(Pt 3):417-39. doi: 10.1042/0264-6021:3530417.
6
Phosphorylation of simian virus 40 T antigen on Thr 124 selectively promotes double-hexamer formation on subfragments of the viral core origin.猿猴病毒40 T抗原在苏氨酸124位点的磷酸化选择性地促进病毒核心起始子亚片段上双六聚体的形成。
J Virol. 2000 Sep;74(18):8601-13. doi: 10.1128/jvi.74.18.8601-8613.2000.
7
Two regions of simian virus 40 T antigen determine cooperativity of double-hexamer assembly on the viral origin of DNA replication and promote hexamer interactions during bidirectional origin DNA unwinding.猴病毒40 T抗原的两个区域决定了双六聚体在病毒DNA复制起点上组装的协同性，并在双向起点DNA解旋过程中促进六聚体相互作用。
J Virol. 1999 Mar;73(3):2201-11. doi: 10.1128/JVI.73.3.2201-2211.1999.
8
Protein phosphatase 2A is required for the initiation of chromosomal DNA replication.蛋白质磷酸酶2A是染色体DNA复制起始所必需的。
Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14693-8. doi: 10.1073/pnas.95.25.14693.
9
Phosphorylation sites in polyomavirus large T antigen that regulate its function in viral, but not cellular, DNA synthesis.多瘤病毒大T抗原中调节其在病毒而非细胞DNA合成中功能的磷酸化位点。
J Virol. 1997 Sep;71(9):6472-8. doi: 10.1128/JVI.71.9.6472-6478.1997.
10
Mechanisms of simian virus 40 T-antigen activation by phosphorylation of threonine 124.苏氨酸124磷酸化激活猿猴病毒40 T抗原的机制。
J Virol. 1996 Jun;70(6):3887-93. doi: 10.1128/JVI.70.6.3887-3893.1996.
猿猴病毒40大T抗原磷酸化位点的代谢周转
J Virol. 1983 Jan;45(1):442-6. doi: 10.1128/JVI.45.1.442-446.1983.
4
Improved localization of phosphorylation sites in simian virus 40 large T antigen.猿猴病毒40大T抗原中磷酸化位点定位的改进
J Virol. 1983 Jan;45(1):315-31. doi: 10.1128/JVI.45.1.315-331.1983.
5
Simian virus 40 large T antigen is phosphorylated at multiple sites clustered in two separate regions.猿猴病毒40大T抗原在聚集于两个不同区域的多个位点发生磷酸化。
J Virol. 1982 Oct;44(1):116-33. doi: 10.1128/JVI.44.1.116-133.1982.
6
Phosphorylation pattern of large T antigens in mouse cells infected by simian virus 40 wild type or deletion mutants.被猿猴病毒40型野生型或缺失突变体感染的小鼠细胞中大T抗原的磷酸化模式。
J Virol. 1982 Sep;43(3):761-71. doi: 10.1128/JVI.43.3.761-771.1982.
7
In vitro mutagenesis of a putative DNA binding domain of SV40 large-T.猴空泡病毒40大T抗原假定DNA结合结构域的体外诱变
Virology. 1984 Nov;139(1):109-37. doi: 10.1016/0042-6822(84)90334-9.
8
Mutations in the phosphorylation sites of simian virus 40 (SV40) T antigen alter its origin DNA-binding specificity for sites I or II and affect SV40 DNA replication activity.猿猴病毒40（SV40）T抗原磷酸化位点的突变会改变其对I或II位点的起始DNA结合特异性，并影响SV40 DNA复制活性。
J Virol. 1988 May;62(5):1598-605. doi: 10.1128/JVI.62.5.1598-1605.1988.
9
Positive and negative regulation of transcription in vitro: enhancer-binding protein AP-2 is inhibited by SV40 T antigen.体外转录的正负调控：增强子结合蛋白AP-2被SV40 T抗原抑制。
Cell. 1987 Sep 11;50(6):847-61. doi: 10.1016/0092-8674(87)90512-5.
10
Regulation of SV40 DNA replication by phosphorylation of T antigen.大T抗原磷酸化对猴病毒40（SV40）DNA复制的调控
EMBO J. 1987 Jan;6(1):153-60. doi: 10.1002/j.1460-2075.1987.tb04733.x.