Li Jie, Zhao Guo-Zhen, Chen Hua-Hong, Qin Sheng, Xu Li-Hua, Jiang Cheng-Lin, Li Wen-Jun
The Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
Syst Appl Microbiol. 2008 Jun;31(2):108-13. doi: 10.1016/j.syapm.2008.03.004. Epub 2008 May 16.
An endophytic actinobacterium, designated YIM 65003(T), was isolated from a surface sterilized leaf sample of Cercidiphyllum japonicum collected from Yunnan province, south-west China. The morphological and chemotaxonomic properties of the isolate were typical of members of the genus Rhodococcus. Analysis of the 16S rRNA gene sequence revealed that the isolate was most closely related to Rhodococcus fascians DSM 20669(T) (99.6%) and Rhodococcus yunnanensis YIM 70056(T) (99.0%). DNA-DNA hybridization with the above microorganisms (46.3% and 48.8%, respectively), in combination with differences in the biochemical and physiological properties, suggested that strain YIM 65003(T) should be classified within a novel species of the genus Rhodococcus, for which the name Rhodococcus cercidiphylli sp. nov. is proposed, with YIM 65003(T) (=CCTCC AB 207160(T)=DSM 45141(T)) as the type strain. The 16S rRNA gene sequence of strain YIM 65003(T) has been deposited in GenBank under the accession number EU325542.
从中国西南部云南省采集的连香树表面消毒叶片样本中分离出一株内生放线菌，命名为YIM 65003(T)。该分离株的形态和化学分类学特性是红球菌属成员的典型特征。16S rRNA基因序列分析表明，该分离株与法氏红球菌DSM 20669(T)（99.6%）和云南红球菌YIM 70056(T)（99.0%）关系最为密切。与上述微生物的DNA-DNA杂交（分别为46.3%和48.8%），结合生化和生理特性的差异，表明菌株YIM 65003(T)应归类为红球菌属的一个新物种，为此提出名为云南红球菌（Rhodococcus cercidiphylli sp. nov.）的新物种，以YIM 65003(T)（=CCTCC AB 207160(T)=DSM 45141(T)）作为模式菌株。菌株YIM 65003(T)的16S rRNA基因序列已保存在GenBank中，登录号为EU325542。
