van Bokhoven H, Mulders M, Wellink J, Vlak J M, Goldbach R, van Kammen A
Department of Molecular Biology, Agricultural University, Wageningen, The Netherlands.
J Gen Virol. 1991 Mar;72 ( Pt 3):567-72. doi: 10.1099/0022-1317-72-3-567.
The poliovirus RNA polymerase has been synthesized in Spodoptera frugiperda cells by using the baculovirus expression system. Crude sonicates of these cells exhibited an RNA-elongating activity of a synthetic oligo(U) primer with poly(A) or cowpea mosaic virus (CPMV) RNA as a template. A similar polymerase activity was found in extracts of insect cells in which foot-and-mouth disease virus (FMDV) proteins, including the putative polymerase, were produced. The analogous CPMV 87K protein and several of its precursors, synthesized in S. frugiperda cells, did not show any detectable polymerase activity in the same assay under a variety of conditions. The results indicate that, in contrast to the picornaviral polymerases, the CPMV polymerase is unable to function in an oligo(U)-primed polymerase assay.
利用杆状病毒表达系统在草地贪夜蛾细胞中合成了脊髓灰质炎病毒RNA聚合酶。这些细胞的粗超声破碎物以聚腺苷酸（poly(A)）或豇豆花叶病毒（CPMV）RNA为模板，表现出对合成寡聚尿苷酸（oligo(U)）引物的RNA延伸活性。在产生包括假定聚合酶在内的口蹄疫病毒（FMDV）蛋白的昆虫细胞提取物中也发现了类似的聚合酶活性。在多种条件下，在草地贪夜蛾细胞中合成的类似CPMV 87K蛋白及其几种前体在相同测定中未显示任何可检测到的聚合酶活性。结果表明，与小核糖核酸病毒聚合酶不同，CPMV聚合酶在寡聚尿苷酸引发的聚合酶测定中无法发挥作用。
