Franklin Vanessa, Khoo Poh Lynn, Bildsoe Heidi, Wong Nicole, Lewis Samara, Tam Patrick P L
Embryology Unit, Children's Medical Research Institute, University of Sydney, Locked Bag 23, Wentworthville, NSW 2145, Australia.
Mech Dev. 2008 Jul;125(7):587-600. doi: 10.1016/j.mod.2008.04.001. Epub 2008 Apr 11.
This fate-mapping study reveals that the progenitors of all major parts of the embryonic gut are already present in endoderm of the early-head-fold to early-somite stage (1-9 somites) mouse embryo. The anterior endoderm contributes primarily to the anterior intestinal portal of the early-organogenesis stage (16-19 somites) embryo. Endoderm cells around and lateral to the node are allocated to the open "midgut" region of the embryonic gut. The posterior (post-nodal) endoderm contributes not only to the posterior intestinal portal but also the open "midgut". Descendants of the posterior endoderm span a length of the gut from the level of the 3rd-5th somites to the posterior end of the embryonic gut. The formation of the anterior and posterior intestinal portals is accompanied by similar repertoires of morphogenetic tissue movement. We also discovered that cells on contralateral sides of the anterior endoderm are distributed asymmetrically to the dorsal and ventral sides of the anterior intestinal portal, heralding the acquisition of laterality by the embryonic foregut.
这项命运图谱研究表明，胚胎肠道所有主要部分的祖细胞在早期头褶到早期体节阶段（1 - 9个体节）的小鼠胚胎内胚层中就已存在。前内胚层主要对早期器官发生阶段（16 - 19个体节）胚胎的前肠门做出贡献。围绕节点及其外侧的内胚层细胞被分配到胚胎肠道的开放“中肠”区域。后（节点后）内胚层不仅对后肠门有贡献，还对开放的“中肠”有贡献。后内胚层的后代跨越从第3 - 5体节水平到胚胎肠道后端的一段肠道长度。前肠门和后肠门的形成伴随着相似的形态发生组织运动模式。我们还发现，前内胚层对侧的细胞不对称地分布在前肠门的背侧和腹侧，预示着胚胎前肠获得了左右不对称性。
