Stein J M, Smith G A, Luzio J P
Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital, U.K.
Biochem J. 1991 Mar 1;274 ( Pt 2)(Pt 2):375-9. doi: 10.1042/bj2740375.
A method for the quantification of membrane lipids by acetylation with [3H]acetic anhydride is described. A standard lipid, labelled with 14C or 32P, is added to the sample and is simultaneously acetylated. The 3H/14C or 3H/32P ratios obtained in the acetylated lipids are proportional to the initial lipid concentration. The method has been used to quantify cholesterol, phosphatidylethanolamine, phosphatidylinositol, sphingomyelin, phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate in amounts between 2 and 10 nmol.
描述了一种用[3H]乙酸酐乙酰化定量膜脂的方法。将用14C或32P标记的标准脂质加入样品中并同时进行乙酰化。乙酰化脂质中获得的3H/14C或3H/32P比值与初始脂质浓度成正比。该方法已用于定量2至10 nmol之间的胆固醇、磷脂酰乙醇胺、磷脂酰肌醇、鞘磷脂、磷脂酰肌醇4-磷酸和磷脂酰肌醇4,5-二磷酸。
