Weerapana Eranthie, Simon Gabriel M, Cravatt Benjamin F
The Skaggs Institute for Chemical Biology and Department of Chemical Physiology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
Nat Chem Biol. 2008 Jul;4(7):405-7. doi: 10.1038/nchembio.91. Epub 2008 May 18.
Insights into the proteome reactivity of electrophiles are crucial for designing activity-based probes for enzymes lacking cognate affinity labels. Here, we show that different classes of carbon electrophiles exhibit markedly distinct amino acid labeling profiles in proteomes, ranging from selective reactivity with cysteine to adducts with several amino acids. These data specify electrophilic chemotypes with restricted and permissive reactivity profiles to guide the design of next-generation functional proteomics probes.
深入了解亲电试剂的蛋白质组反应性对于设计针对缺乏同源亲和标记的酶的基于活性的探针至关重要。在此，我们表明不同类别的碳亲电试剂在蛋白质组中表现出明显不同的氨基酸标记谱，范围从与半胱氨酸的选择性反应到与多种氨基酸的加合物。这些数据确定了具有受限和允许反应谱的亲电化学类型，以指导下一代功能蛋白质组学探针的设计。
