Takeuchi Paula Lumy, Antunes Lusânia Maria Greggi, Takahashi Catarina Satie
Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil.
Arch Toxicol. 2008 Nov;82(11):869-73. doi: 10.1007/s00204-008-0308-8. Epub 2008 May 17.
Vitamin B(6) has shown to be a potentially effective antioxidant agent, and dietary antioxidants are also frequently valuable inhibitors of clastogenesis and carcinogenesis. The purpose of the present work was to study the clastogenicity of different doses of vitamin B(6) and to examine the possible modulating effect of this vitamin on chromosomal damage induced by the antitumor agent doxorubicin in Wistar rats. Experimental groups were set up for pre- and simultaneous treatment with vitamin B(6) alone or in combination with DXR. The data obtained from administering different doses of vitamin B(6) (12.5-100 mg/kg b.w.) showed no significant increase in total chromosomal aberrations when compared with the negative control. The administration of two doses of 25 mg/kg b.w. or one dose of 50 mg/kg b.w. of vitamin B(6) before doxorubicin injection seemed equally effective in protecting cells against doxorubicin clastogenicity. The anticlastogenic effect of vitamin B(6) on DXR-induced chromosomal damage could be ascribed to its antioxidant properties. Vitamin B(6) was not clastogenic or cytotoxic in rat bone marrow cells and it plays a role in inhibiting the clastogenicity induced by DXR.
维生素B6已被证明是一种潜在有效的抗氧化剂，而膳食抗氧化剂通常也是染色体断裂和致癌作用的重要抑制剂。本研究的目的是研究不同剂量维生素B6的染色体断裂效应，并考察该维生素对Wistar大鼠体内抗肿瘤药物阿霉素诱导的染色体损伤的可能调节作用。设立了单独使用维生素B6或与阿霉素联合使用进行预处理和同时处理的实验组。给予不同剂量维生素B6（12.5 - 100mg/kg体重）的数据显示，与阴性对照组相比，总染色体畸变没有显著增加。在注射阿霉素前给予两剂25mg/kg体重或一剂50mg/kg体重的维生素B6似乎在保护细胞免受阿霉素染色体断裂效应方面同样有效。维生素B6对阿霉素诱导的染色体损伤的抗断裂效应可归因于其抗氧化特性。维生素B6在大鼠骨髓细胞中不具有染色体断裂效应或细胞毒性，并且在抑制阿霉素诱导的染色体断裂效应中发挥作用。
