Jia Rui-Peng, Lin Jian-Zhong, Liu Jun, Su Jiang-Hao, Bao Qing-Bing, Zhu Jia-Geng
Department of Urology, the First Hospital of Nanjing Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210006, China.
Zhonghua Nan Ke Xue. 2008 Mar;14(3):220-3.
To investigate the effect of PI-3K and p38MAPK signal pathways on the cyclooxygenase-2 (COX-2) expression induced by the epidermal growth factor (EGF) in PC-3 cells.
PC-3 cell proliferation was detected by methylthiazolyl tetrazolium (MTT) assay after stimulated by EGF (0 microg/L), EGF (10 microg/L), EGF (10 microg/L) + LY294002 (20 micromol/L) and EGF (10 microg/L) + SC203580 (20 micromol/L), and so was the COX-2 expression in the PC-3 cells by RT-PCR and Western blot assay after stimulated the same way for 24 hours. ELISA was used to determine the changes of PGE2 in the culture medium.
LY294002 and SC203580 signficantly inhibited PC-3 cell proliferation (P < 0.05), COX-2 expression and PGE2 production after EGF stimulation (P < 0.05).
EGF can stimulate PC-3 cells into proliferation and induce COX-2 mRNA and the upregulation of its protein expression, while LY294002 and SC203580 can inhibit EGF from the above effects on PC-3 cells.
探讨磷脂酰肌醇-3激酶（PI-3K）和p38丝裂原活化蛋白激酶（p38MAPK）信号通路对表皮生长因子（EGF）诱导的PC-3细胞中环氧合酶-2（COX-2）表达的影响。
采用噻唑蓝（MTT）法检测表皮生长因子（0μg/L）、表皮生长因子（10μg/L）、表皮生长因子（10μg/L）+LY294002（20μmol/L）和表皮生长因子（10μg/L）+SC203580（20μmol/L）刺激后PC-3细胞的增殖情况，采用逆转录-聚合酶链反应（RT-PCR）和蛋白质免疫印迹法（Western blot）检测以同样方式刺激24小时后PC-3细胞中COX-2的表达情况。采用酶联免疫吸附测定法（ELISA）检测培养基中前列腺素E2（PGE2）的变化。
LY294002和SC203580显著抑制表皮生长因子刺激后PC-3细胞的增殖（P<0.05）、COX-2表达及PGE2生成（P<0.05）。
表皮生长因子可刺激PC-3细胞增殖，诱导COX-2 mRNA及其蛋白表达上调，而LY294002和SC203580可抑制表皮生长因子对PC-3细胞的上述作用。
