[转化生长因子β上调前列腺癌LNCaP细胞系中侵袭和转移相关蛋白的表达（体外实验）]
[Transforming growth factor beta upregulates the expression of invasion and metastasis associated proteins in prostate cancer LNCaP cell lines in vitro].
作者信息
Zhu Guang-Feng, Cheng He-Peng, Wu Kai-Jie, Zhang Lin-Lin, Zhu Guo-Dong, Zhang Dong, He Da-Lin
机构信息
Urology Institute of Xi'an Jiaotong University, Department of Urology, the First Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi 710061, China.
出版信息
Zhonghua Nan Ke Xue. 2008 Mar;14(3):238-41.
OBJECTIVE
To determine the effect of the transforming growth factor beta (TGF-beta) on the expression of invasion and metastasis associated proteins in the prostate cancer LNCaP cell line in vitro.
METHODS
The prostate cancer cell line LNCaP was treated with TGF-beta in vitro. Western blotting was used to detect the expression of the "invasion and metastasis" associated proteins E-Cadherin, N-Cadherin and Vimentin.
RESULTS
The expression of N-Cadherin and Vimentin of the LNCaP cells treated with TGF-beta for 12 hours was significantly upregulated, but not that of E-Cadherin.
CONCLUSION
TGF-beta may induce epithelial-mesenchymal transition (EMT) of LNCaP cells which might be of importance in promoting prostate cancer cells invading to ambient tissues and metastasizing to distant organs.
目的
确定转化生长因子β（TGF-β）对前列腺癌LNCaP细胞系体外侵袭和转移相关蛋白表达的影响。
方法
体外使用TGF-β处理前列腺癌细胞系LNCaP。采用蛋白质免疫印迹法检测“侵袭和转移”相关蛋白E-钙黏蛋白、N-钙黏蛋白和波形蛋白的表达。
结果
用TGF-β处理12小时的LNCaP细胞中，N-钙黏蛋白和波形蛋白的表达显著上调，但E-钙黏蛋白的表达未上调。
结论
TGF-β可能诱导LNCaP细胞发生上皮-间质转化（EMT），这可能在促进前列腺癌细胞侵袭周围组织和转移至远处器官方面具有重要意义。
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