Kas'ianenko N A, Zakharova N B, Mukhin D A, Slita A V, Nazarova O V, Leont'eva E A, Panarin E F
Biofizika. 2008 Jan-Feb;53(1):48-54.
The formation and physicochemical properties of high-molecular thymus and plasmid DNA complexes with synthetic polymers based on (dimethyl-amino)ethyl methacrylate (DMAEM), (diethyl-amino)ethyl methacrylate (DEAEM), and polyvinyl amine (PVA) were investigated in solutions of different ionic strength by low-gradient viscometry, electrophoresis, circular dichroism, spectrophotometry, and dynamic light scattering. The toxicity of complexes in T98G cells was studied. It was shown that, when the ratio of polycations to DNA charged groups concentration (N+/P) reaches values > 1, DNA condensation occurs. It is accompanied by increasing optical density of solutions. Changes in DNA size after condensation were estimated. Phase diagrams of systems DNA/polycation in the presence of NaCl were obtained. It was shown by MTT-analysis that DNA complexes with polycations in the range of concentrations used have low toxicity.
通过低梯度粘度测定法、电泳、圆二色性、分光光度法和动态光散射，研究了基于甲基丙烯酸（二甲氨基）乙酯（DMAEM）、甲基丙烯酸（二乙氨基）乙酯（DEAEM）和聚乙烯胺（PVA）的高分子胸腺和质粒DNA复合物与合成聚合物在不同离子强度溶液中的形成及物理化学性质。研究了复合物在T98G细胞中的毒性。结果表明，当聚阳离子与DNA带电基团浓度之比（N+/P）达到>1的值时，会发生DNA凝聚。这伴随着溶液光密度的增加。估计了凝聚后DNA大小的变化。获得了在NaCl存在下DNA/聚阳离子体系的相图。MTT分析表明，在所使用的浓度范围内，DNA与聚阳离子的复合物具有低毒性。
