Kasyanenko Nina, Dribinsky Boris
Department of Physics, St. Petersburg State University, 7/9 Universitetskaya nab., St. Petersburg 199034, Russia.
Department of Physics, St. Petersburg State University, 7/9 Universitetskaya nab., St. Petersburg 199034, Russia.
Int J Biol Macromol. 2016 May;86:216-23. doi: 10.1016/j.ijbiomac.2016.01.044. Epub 2016 Jan 15.
A comparison of DNA conformational changes in a solution containing the poly-l-lysine with the number of monomers z=3, 5, 17, 20, 270, 325 and polyamines (spermine and spermidine) was carried out in 1M and 5mM NaCl solutions. It was shown that despite the identical results of DNA condensation induced by compounds, their influence on the DNA conformation prior to packaging depends on whether they belong to a long polycations or short oligomers. DNA secondary and tertiary structures were examined using Circular Dichroism, UV-vis Spectrophotometry, Dynamic Light Scattering, Low Gradient Viscometry, Flow Birefringence, and AFM. The phase diagrams for systems of DNA-polycations, DNA-oligomers, DNA-polyamines were drawn.
在1M和5mM NaCl溶液中，对含有单体数量z = 3、5、17、20、270、325的聚-L-赖氨酸溶液以及多胺（精胺和亚精胺）中的DNA构象变化进行了比较。结果表明，尽管这些化合物诱导DNA凝聚的结果相同，但它们在包装前对DNA构象的影响取决于它们属于长链聚阳离子还是短链寡聚物。使用圆二色性、紫外可见分光光度法、动态光散射、低梯度粘度测定法、流动双折射和原子力显微镜对DNA的二级和三级结构进行了检测。绘制了DNA-聚阳离子、DNA-寡聚物、DNA-多胺体系的相图。
