Nagai Toshihiro, Sato Masato, Furukawa Katsuko S, Kutsuna Toshiharu, Ohta Naoshi, Ushida Takashi, Mochida Joji
Department of Orthopedic Surgery and Surgical Science, School of Medicine, Tokai University, Kanagawa, Japan.
Tissue Eng Part A. 2008 Jul;14(7):1225-35. doi: 10.1089/ten.tea.2007.0225.
If a tissue-engineered cartilage transplant is to succeed, it needs to integrate with the host tissue, to endure physiological loading, and to acquire the phenotype of the articular cartilage. Although there are many reported treatments for osteochondral defects of articular cartilage, problems remain with the use of artificial matrices (scaffolds) and the stage of implantation. We constructed scaffold-free three-dimensional tissue-engineered cartilage allografts using a rotational culture system and investigated the optimal stage of implantation and repair of the remodeling site. We evaluated the amounts of extracellular matrix and gene expression levels in scaffold-free constructs and transplanted the constructs for osteochondral defects using a rabbit model. Allografted 2-week constructs expressed high levels of proteoglycan and collagen per DNA content, integrated with the host cartilage successfully, and were able to counter physiological loads, and the chondrocyte plate contributed reparative mesenchymal stem cells to the final phenotype of the articular cartilage.
若组织工程化软骨移植要取得成功，它需要与宿主组织整合，承受生理负荷，并获得关节软骨的表型。尽管已有许多关于关节软骨骨软骨缺损的治疗报道，但在人工基质（支架）的使用和植入阶段仍存在问题。我们使用旋转培养系统构建了无支架三维组织工程软骨同种异体移植物，并研究了植入的最佳阶段和重塑部位的修复情况。我们评估了无支架构建体中细胞外基质的量和基因表达水平，并使用兔模型将构建体移植到骨软骨缺损处。移植2周的构建体每DNA含量表达高水平的蛋白聚糖和胶原蛋白，成功地与宿主软骨整合，并且能够抵抗生理负荷，软骨细胞板为关节软骨的最终表型贡献了修复性间充质干细胞。
