Darley W M, Smiley R H
Can J Microbiol. 1976 Oct;22(10):1502-8. doi: 10.1139/m76-222.
Cultures of Cylindrotheca furisormis grown either autotrohpically or heterotrophically on lactate contained significant amounts of NAD-dependent L(+)-lactate dehydrogenase (EC 1.1.1.27). Polyacylamide gel electrophoresis of crude enzyme extracts revealed a single band which was indistinguishable between autotrohpic and heterotrohpic cells. The Km for lactate of partially purified preparations was lower under heterotrophic conditions. The specific activity in crude extracts was higher under autotrophic than heterotrophic conditions; it dropped precipitously when autotrophic cells were transferred to the dark, increasing again only in the presence of lactate. These and related observations suggest that this enzyme has at most only a minor role in the assimilation of lactate during heterotrophic growth on lactate.
在乳酸上进行自养或异养生长的圆柱鞘丝藻培养物中含有大量的NAD依赖性L(+)-乳酸脱氢酶（EC 1.1.1.27）。粗酶提取物的聚丙烯酰胺凝胶电泳显示出一条单一的条带，自养细胞和异养细胞之间没有区别。部分纯化制剂对乳酸的Km在异养条件下较低。粗提取物中的比活性在自养条件下高于异养条件；当自养细胞转移到黑暗中时，其比活性急剧下降，只有在有乳酸存在时才会再次增加。这些以及相关的观察结果表明，这种酶在以乳酸为底物的异养生长过程中对乳酸同化作用至多只起次要作用。
