Sandgren M, Danforth L, Plasse T F, DeLuca H F
Department of Biochemistry, University of Wisconsin-Madison 53706.
Cancer Res. 1991 Apr 15;51(8):2021-4.
1,25-Dihydroxyvitamin D3 [1,25-(OH)2D3] receptor concentration was measured by an accurate immunoradiometric assay in primary tumors from 10 patients with colorectal carcinoma and 11 patients with non-small cell carcinoma of the lung. Measurements were also performed on a noncancerous sample of the same origin as the tumor from each patient. All of the tumors contained receptor with a mean concentration of 123.4 fmol/mg protein for colorectal and 75.1 fmol/mg protein for lung carcinoma. Compared to normal tissue from the same patient, 100% of the lung tumors and 70% of the colorectal tumors had significantly higher levels of the 1,25-dihydroxyvitamin D3 receptor. A correlation was found between well-differentiated colorectal tumors with no or few metastases and high levels of 1,25-dihydroxyvitamin D3 receptor. Receptor concentration was also assayed in metastatic lesions of malignant melanoma from 7 patients. 1,25-Dihydroxyvitamin D3 receptor was present in 85% of the metastases at a mean level of 26 fmol/mg protein. For these patients an inverse correlation was found between receptor level and age. The results obtained in this pilot study suggest that an alteration in 1,25-dihydroxyvitamin D3 receptor regulation may occur in vivo when a cell undergoes malignant transformation.
采用精确的免疫放射分析方法，对10例结肠直肠癌患者和11例非小细胞肺癌患者的原发性肿瘤中1,25 - 二羟维生素D3 [1,25-(OH)2D3] 受体浓度进行了测定。同时，对每位患者与肿瘤来源相同的非癌性样本也进行了测量。所有肿瘤均含有受体，结肠直肠癌肿瘤中受体的平均浓度为123.4 fmol/mg蛋白质，肺癌肿瘤中为75.1 fmol/mg蛋白质。与同一患者的正常组织相比，100%的肺癌肿瘤和70%的结肠直肠癌肿瘤中1,25 - 二羟维生素D3受体水平显著更高。在无转移或转移较少的高分化结肠直肠癌肿瘤与高水平的1,25 - 二羟维生素D3受体之间发现了相关性。还对7例恶性黑色素瘤转移灶中的受体浓度进行了测定。85%的转移灶中存在1,25 - 二羟维生素D3受体，平均水平为26 fmol/mg蛋白质。对于这些患者，发现受体水平与年龄呈负相关。在这项初步研究中获得的结果表明，当细胞发生恶性转化时，体内1,25 - 二羟维生素D3受体调节可能会发生改变。
