Bruce-Gregorios J H, Soucy D M, Chen M G, Norenberg M D
Department of Pathology, University of Miami-Jackson Memorial Hospital, Florida.
J Neuropathol Exp Neurol. 1991 Mar;50(2):118-25. doi: 10.1097/00005072-199103000-00003.
We have recently reported that methotrexate (MTX) causes degenerative as well as reactive-like astroglial changes and alters the cell cycle kinetics of astrocytes in vitro. To further characterize the nature of the reactive-like changes that were noted by light and electron microscopy following MTX exposure, the glial fibrillary acidic protein (GFAP) content of astrocytes in culture was investigated by enzyme-linked immunosorbent assay, flow cytometry and double-immunofluorescent staining. An increase in GFAP content which did not correlate with drug dosage or DNA synthesis was noted in the MTX-treated cultures. It is postulated that this increase in GFAP content of astrocytes reflects an adaptive response to MTX-induced injury and partly explains the gliosis that is seen in methotrexate encephalopathy.
我们最近报道，甲氨蝶呤（MTX）在体外可引起退行性以及反应样星形胶质细胞变化，并改变星形胶质细胞的细胞周期动力学。为了进一步明确MTX暴露后通过光镜和电镜观察到的反应样变化的性质，我们通过酶联免疫吸附测定、流式细胞术和双重免疫荧光染色研究了培养的星形胶质细胞中胶质纤维酸性蛋白（GFAP）的含量。在MTX处理的培养物中，观察到GFAP含量增加，且该增加与药物剂量或DNA合成无关。据推测，星形胶质细胞中GFAP含量的这种增加反映了对MTX诱导损伤的适应性反应，部分解释了甲氨蝶呤脑病中所见的胶质增生。
