Hughes A L, Dahiya J P, Wyatt C L, Classen H L
Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Poult Sci. 2008 Jun;87(6):1156-61. doi: 10.3382/ps.2007-00505.
Microbial phytase is a prominent feed enzyme used in animal feeds, but there is relatively little information on its use in laying hen diets. In this experiment, an Escherichia coli 6-phytase (Quantum) was evaluated for its efficacy in a 40-wk laying hen production trial. A total of 1,080 White Leghorn hens (540 each of Shaver and Bovan strains) were fed mash corn-soybean meal-based diets containing 0.35% (positive control, PC), 0.25% (negative control, NC1), or 0.15% (NC2) nonphytate phosphorus (NPP). Six more diets were manufactured by supplementing the negative control diets with 200, 400, and 600 U/kg of exogenous phytase, resulting in a total of 9 treatments. Each dietary treatment x strain subclass was replicated 4 times with 5 adjoining cages per replicate (3 hens per cage) in a randomized complete block design. Production performance was measured from 21 to 61 wk of age. Only minor differences in production characteristics were found between the PC and NC1 treatments regardless of phytase addition, indicating that 0.25% NPP resulted in P intake that was at or above the hen's requirement. In contrast, the hens fed 0.15% NPP diet without phytase supplementation had significantly (P < 0.05) reduced total hen housed egg production and body weight at 61 wk of age in comparison to the PC treatment, whereas the incidence of soft-shelled, cracked, and broken eggs was increased significantly (P < 0.05) in hens fed the NC2 diet. Addition of phytase to the NC2 diet improved these production characteristics to levels equal or better than the PC diet. The results indicated that Quantum phytase was efficacious in corn-soybean meal-based diets fed to White Leghorn laying hens and can be used to reduce diet supplementation with inorganic phosphorus.
微生物植酸酶是动物饲料中使用的一种重要的饲料酶,但关于其在蛋鸡日粮中的应用信息相对较少。在本试验中,对一种大肠杆菌6 - 植酸酶(量子)在40周龄蛋鸡生产试验中的效果进行了评估。总共1080只白来航蛋鸡(每个品种540只,分别为沙维玛和博万品种)被饲喂以玉米 - 豆粕为基础的粉料日粮,日粮中含0.35%(阳性对照,PC)、0.25%(阴性对照1,NC1)或0.15%(阴性对照2,NC2)的非植酸磷(NPP)。通过在阴性对照日粮中添加200、400和600 U/kg的外源植酸酶,又配制了6种日粮,从而形成了总共9种处理。在随机完全区组设计中,每个日粮处理×品种亚类重复4次,每个重复有5个相邻的笼子(每个笼子3只母鸡)。在21至61周龄期间测定生产性能。无论是否添加植酸酶,PC和NC1处理之间在生产特性方面仅发现微小差异,这表明0.25%的NPP导致的磷摄入量达到或高于母鸡的需求。相比之下,与PC处理相比,未添加植酸酶而饲喂0.15% NPP日粮的母鸡在61周龄时总存栏蛋产量和体重显著降低(P < 0.05),而饲喂NC2日粮母鸡的软壳蛋、裂纹蛋和破蛋发生率显著增加(P < 0.05)。在NC2日粮中添加植酸酶可将这些生产特性提高到与PC日粮相当或更好的水平。结果表明,量子植酸酶在饲喂白来航蛋鸡的玉米 - 豆粕型日粮中是有效的,可用于减少无机磷的日粮添加量。